Lymphocyte Function-Associated Antigen-1 Expression on Plasma Cells Correlates With Tumor Growth in Multiple Myeloma

• Published in: Blood 1992-04, Vol.79 (8), p.2068-2075
• Main Authors: Ahsmann, Els J.M., Lokhorst, Henk M., Dekker, Ad W., Bloem, Andries C.
• Format: Article
• Language: English
• Subjects: Antibodies, Monoclonal; Antigens, CD - analysis; Biological and medical sciences; Biomarkers, Tumor - analysis; Bone Marrow - immunology; Bone Marrow - pathology; Cytotoxicity, Immunologic; Female; Fluorescent Antibody Technique; Follow-Up Studies; Hematologic and hematopoietic diseases; Hematopoietic Stem Cells - immunology; Hematopoietic Stem Cells - pathology; Humans; Immunoglobulins - analysis; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lymphocyte Function-Associated Antigen-1 - analysis; Male; Medical sciences; Middle Aged; Mitotic Index; Molecular Weight; Multiple Myeloma - immunology; Multiple Myeloma - pathology; Phenotype; Reference Values; T-Lymphocytes - immunology; T-Lymphocytes - pathology
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V79.8.2068.2068

Lymphocyte function-associated antigen-1 (LFA-1) (CD11a/ CD18) expression on bone marrow-derived plasma cells from normal individuals, patients with monoclonal gammo-pathies of undetermined significance (MGUS), and patients with multiple myeloma (MM) was studied by immunofluorescence microscopy and flow cytometry using a new mono-clonal antibody (MoAb) F8.8. This MoAb recognizes the α-chain (CD11a) of LFA-1 as determined by immunoprecipita-tion, and inhibits T-cell-induced cytotoxicity. Although the F8.8 MoAb stains unstimulated peripheral blood T cells with the same mean fluorescence intensity as other anti-CD11a MoAbs, it proved to be superior in detecting CD11a on plasma cells as compared with reference MoAbs. Using the anti-CD11a MoAb F8.8, a strong correlation was found be- tween LFA-1 expression and disease activity in MM, as defined by clinical performance and serum M-protein level. Hardly any LFA-1+ plasma cells were detected in normal individuals, patients with MGUS, and MM patients in a nonactive phase of their disease, while plasma cells of some MM patients with active disease and all patients with fulminant disease expressed LFA-1. Plasma cell LFA-1 expression correlated well with the labeling index (LI) of the tumors in the individual patients. The relation between LFA-1 expression and the tumor growth suggests an involvement of this adhesion molecule in cellular interactions resulting in plasma cell proliferation. © 1992 by The American Society of Hematology.