Comparison of herpesviruses isolated from reindeer, goats, and cattle by restriction endonuclease analysis

A genomic comparison of bovine herpesvirus 1 (BHV-1), caprine herpesvirus (CHV-2) and reindeer herpesvirus (RHV), was performed using 5 restriction endonucleases. Cross neutralization of these three herpesviruses showed that BHV-1 and CHV-2 had a relatively low degree of cross reaction with heterolo...

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Bibliographic Details
Published in:Archives of virology 1992-01, Vol.123 (3-4), p.389-397
Main Authors: RIMSTAD, E, KRONA, R, HYLLSETH, B
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cattle
Cells, Cultured
Cross Reactions
DNA Restriction Enzymes - metabolism
Female
Fundamental and applied biological sciences. Psychology
Genes. Genome
Goats - microbiology
Herpesviridae
Herpesviridae - genetics
Herpesviridae - immunology
Herpesviridae - isolation & purification
Herpesviridae Infections - microbiology
Herpesviridae Infections - veterinary
herpesvirus
Herpesvirus 1, Bovine - genetics
Male
Molecular and cellular biology
Molecular genetics
Neutralization Tests
Rangifer tarandus tarandus
Reindeer - microbiology
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Summary:A genomic comparison of bovine herpesvirus 1 (BHV-1), caprine herpesvirus (CHV-2) and reindeer herpesvirus (RHV), was performed using 5 restriction endonucleases. Cross neutralization of these three herpesviruses showed that BHV-1 and CHV-2 had a relatively low degree of cross reaction with heterologous viruses. RHV showed a higher degree of such cross reactivity. The restriction endonuclease analyses showed that the migration patterns of the DNA segments were different for the three groups of herpesviruses. The enteric caprine strain could be differentiated from genital strains using BstE II and Hpa I. The genome size of reindeer herpesvirus was estimated to be approximately 86.8 x 10(6) Da (131.8 kbp), and indications of isomerization of this genome were found. It is concluded that reindeer herpesvirus is a distinct species within the family Herpesviridae.
ISSN:0304-8608
1432-8798
DOI:10.1007/BF01317272