LC-NMR applied to the characterisation of cardiac glycosides from three micropropagated Isoplexis species

Species of the genus Isoplexis are of particular interest with respect to the biochemical pathway leading to the cardenolides. It is important to determine whether or not 5β‐configured compounds, typically produced by Digitalis species and used in medicine, are present together with their respective...

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Bibliographic Details
Published in:Phytochemical analysis 2002-09, Vol.13 (5), p.266-271
Main Authors: Gavidia, I, Seitz, H.U, Perez-Bermudez, P, Vogler, B
Format: Article
Language:English
Subjects:
aglycone
canarigenin
cardiac glycosides
Cardiac Glycosides - chemistry
Cardiac Glycosides - isolation & purification
cardiovascular agents
chemical structure
Chromatography, Liquid - methods
Culture Techniques
Digitalis
digitoxigenin
high performance liquid chromatography
Isoplexis
Isoplexis canariensis
Isoplexis chalcantha
Isoplexis isabelliana
LC-NMR
leaves
Magnetic Resonance Spectroscopy - methods
medicinal plants
micropropagation
Molecular Structure
nuclear magnetic resonance spectroscopy
phytochemicals
plant extracts
pregnane glycosides
Scrophulariaceae
Scrophulariaceae - chemistry
secondary metabolic pathways
secondary metabolites
uzarigenin
xysmalogenin
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Summary:Species of the genus Isoplexis are of particular interest with respect to the biochemical pathway leading to the cardenolides. It is important to determine whether or not 5β‐configured compounds, typically produced by Digitalis species and used in medicine, are present together with their respective α‐isomers in Isoplexis spp. Structure elucidation by LC‐NMR of the products isolated from in vitro regenerated Isoplexis canariensis, I. chalcantha and I. isabelliana was carried out, and similarities were observed among the products of the three species, including the presence of digitoxigenin‐type cardenolides in I. canariensis and xysmalogenin and canarigenin derivatives in I. chalcantha never previously reported in these species. Pregnane glycosides not found until now either in Isoplexis or Digitalis were also detected. Copyright © 2002 John Wiley & Sons, Ltd.
ISSN:0958-0344
1099-1565
DOI:10.1002/pca.655