Umbilical Cord Blood: the Role of Apoptosis in the Control of CD34+ Cell Counts

BACKGROUND AND OBJECTIVE: Cord blood obtained at delivery can be used for hematopoietic precursor cells (HPC) transplantation. The major limit for its success is represented by the low cellular yield of the stem cell population. The objective of this study was to determine the role played by apoptos...

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Bibliographic Details
Published in:Placenta (Eastbourne) 2003-01, Vol.24 (1), p.113-115
Main Authors: Mastino, A., Favalli, C., Camilli, A.R., Malerba, C., Grelli, S., Calugi, A.
Format: Article
Language:English
Subjects:
Adult
Annexin A5 - metabolism
Antigens, CD34 - blood
Apoptosis - physiology
Biological and medical sciences
Cell Separation
Cells, Cultured
Female
Fetal Blood - cytology
Fetal Blood - physiology
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - physiology
Humans
Leukocytes, Mononuclear - cytology
Lymphocyte Count
Mother. Fetoplacental unit. Mammary gland. Milk
Phosphatidylserines - analysis
Phosphatidylserines - metabolism
Pregnancy
Pregnancy. Parturition. Lactation
Time Factors
Vertebrates: reproduction
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Summary:BACKGROUND AND OBJECTIVE: Cord blood obtained at delivery can be used for hematopoietic precursor cells (HPC) transplantation. The major limit for its success is represented by the low cellular yield of the stem cell population. The objective of this study was to determine the role played by apoptosis in the numerical control of CD34+ cell counts. DESIGN AND METHODS: Umbilical cord blood samples were collected from 15 women at the time of the delivery and cord blood units processed. Cells, collected following 24h and 48h of incubation, were analysed by flow cytometry using the gating strategy. RESULTS: Remarkable levels of apoptosis were detected in the stem cell population and a significant difference between apoptosis mean values at 24h and 48h within CD34+ cells were found. The difference between the percentage of apoptosis in CD34+ cells and that in the remaining population was significant both at 24h and at 48h. CONCLUSIONS: CD34+ cells have a higher likelihood to undergo apoptosis in comparison to the remaining ones present in umbilical cord blood. This process of cellular death plays a major role in the control of CD34+ cell counts in placental blood and influence, for this reason, the possibility of success of a cord blood transplantation.
ISSN:0143-4004
1532-3102
DOI:10.1053/plac.2002.0873