High-performance liquid chromatographic stationary phases based on poly(methyloctylsiloxane) immobilized on silica: II. Chromatographic evaluation

• Published in: Journal of Chromatography A 2002-03, Vol.948 (1), p.109-119
• Main Authors: Tonhi, Edivan, Collins, Kenneth E., Collins, Carol H.
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Carbon - chemistry; Chemical Phenomena; Chemistry; Chemistry, Physical; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid; Exact sciences and technology; Gamma Rays; Indicators and Reagents; Microwaves; Other chromatographic methods; Poly(methyloctylsiloxane); Polymers - chemistry; Silicon Dioxide - chemistry; Siloxanes - chemistry; Solvents; Temperature
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(02)00006-7

This work describes the chromatographic characterization of stationary phases prepared by deposition of poly(methyloctylsiloxane) (PMOS) on silica followed by immobilization using one of several different processes: thermal treatments (120 or 220 °C for 4 h), microwave irradiation (495 W for 15 min), γ radiation (dose of 80 kGy) or self-immobilization. This evaluation was based on the chromatographic parameters of several test solutes. The stationary phases immobilized at 220 °C and which underwent self-immobilization were not appropriate for chromatographic use but the other immobilized phases presented chromatographic performances similar in most respects to a commercial phase (Rainin C 8) while the peak characteristics of the basic probe were significantly better with these phases.