Sequence and conservation of a rRNA and tRNAVal mitochondrial gene fragment from Penaeus californiensis and comparison with Penaeus vannamei and Penaeus stylirostris

Penaeus californiensis is an important species for shrimp fisheries in the Pacific Ocean and has recently been described as a potential cultured species, mainly through the winter season in subtropical regions. A fragment of the mitochondrial 12S rRNA-tRNAVal-16S rRNA genes from P. californiensis wa...

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Bibliographic Details
Published in:Marine biotechnology (New York, N.Y.) N.Y.), 2002-09, Vol.4 (4), p.392-398
Main Authors: Gutiérrez-Millán, Luis Enrique, Peregrino-Uriarte, Alma Beatriz, Sotelo-Mundo, Rogerio, Vargas-Albores, Francisco, Yepiz-Plascencia, Gloria
Format: Article
Language:English
Subjects:
Conserved sequence
Deoxyribonucleic acid
DNA
Fisheries
Fragmentation
Genes
Genetic distance
Marine crustaceans
Mitochondrial DNA
Nucleotide sequence
PCR
Polymerase chain reaction
Primers
Regions
rRNA 12S
rRNA 16S
Sequencing
Shellfish
Shrimp fisheries
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Summary:Penaeus californiensis is an important species for shrimp fisheries in the Pacific Ocean and has recently been described as a potential cultured species, mainly through the winter season in subtropical regions. A fragment of the mitochondrial 12S rRNA-tRNAVal-16S rRNA genes from P. californiensis was sequenced and compared with the corresponding regions from Penaeus vannamei and Penaeus stylirostris. Purified mitochondrial DNA was used for polymerase chain reaction amplification with primers for 12S and 16S rRNA genes. A 1379 +/- 1-bp fragment was obtained, including 90% 16S rRNA, tRNAVal, and a portion of 12S rRNA, cloned, and sequenced. Genetic distances were calculated according to the Kimura 2-parameter distance model, and maximum-likelihood analysis was applied with 1000 bootstrap replications. Sequence identity of P. californiensis with both P. vannamei and P. stylirostris was 0.88, while for P. vannamei and P. stylirostris the identity was 0.92. Maximum-likelihood analysis grouped P. vannamei and P. stylirostris separately from P. californiensis.
ISSN:1436-2228
1436-2236
DOI:10.1007/s10126-002-0008-y