Polyethyleneimine/chitosan hexamer-mediated gene transfection into intestinal epithelial cell cultured in serum-containing medium

In search of an efficient nonviral vector, polyethyleneimine (PEI)-based vectors were examined. In general, the transfection efficiency of nonviral vectors is suppressed by serum. Here we show that PEI based vectors, particularly, the chitosan hexamer-PEI vector, could perform efficient gene transfe...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2002-07, Vol.94 (1), p.81-83
Main Authors: Ouji, Yukiteru, Yoshida-Terakura, Ayako, Hayashi, Yuka, Maeda, Isamu, Kawase, Masaya, Yamato, Eiji, Miyazaki, Jun-ichi, Yagi, Kiyohito
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
CELL CULTURE
CHITOSAN
EPITHELIUM
Fundamental and applied biological sciences. Psychology
GENES
Genetic engineering
Genetic technics
INTESTINES
Methods. Procedures. Technologies
nonviral vector
PEI
serum-containing medium
TRANSFECTION
Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Summary:In search of an efficient nonviral vector, polyethyleneimine (PEI)-based vectors were examined. In general, the transfection efficiency of nonviral vectors is suppressed by serum. Here we show that PEI based vectors, particularly, the chitosan hexamer-PEI vector, could perform efficient gene transfection into intestinal epithelial cells (IEC-6) in the presence of serum. The conjugation order of the two polymers with a plasmid (first, chitosan hexamer; second, PEI) was found to be an important factor in enhancing transfection efficiency.
ISSN:1389-1723
1347-4421
DOI:10.1016/S1389-1723(02)80121-9