Polyethyleneimine/chitosan hexamer-mediated gene transfection into intestinal epithelial cell cultured in serum-containing medium

In search of an efficient nonviral vector, polyethyleneimine (PEI)-based vectors were examined. In general, the transfection efficiency of nonviral vectors is suppressed by serum. Here we show that PEI based vectors, particularly, the chitosan hexamer-PEI vector, could perform efficient gene transfe...

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Published in:Journal of bioscience and bioengineering 2002-07, Vol.94 (1), p.81-83
Main Authors: Ouji, Yukiteru, Yoshida-Terakura, Ayako, Hayashi, Yuka, Maeda, Isamu, Kawase, Masaya, Yamato, Eiji, Miyazaki, Jun-ichi, Yagi, Kiyohito
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
CELL CULTURE
CHITOSAN
EPITHELIUM
Fundamental and applied biological sciences. Psychology
GENES
Genetic engineering
Genetic technics
INTESTINES
Methods. Procedures. Technologies
nonviral vector
PEI
serum-containing medium
TRANSFECTION
Vectors (cloning, transfer, expression). Insertion sequences and transposons
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cited_by cdi_FETCH-LOGICAL-c3434-f5b2c2ef9837f989d894853d60a798bd83173e961cc140181a11d9002b5104343
cites cdi_FETCH-LOGICAL-c3434-f5b2c2ef9837f989d894853d60a798bd83173e961cc140181a11d9002b5104343
container_end_page 83
container_issue 1
container_start_page 81
container_title Journal of bioscience and bioengineering
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creator Ouji, Yukiteru
Yoshida-Terakura, Ayako
Hayashi, Yuka
Maeda, Isamu
Kawase, Masaya
Yamato, Eiji
Miyazaki, Jun-ichi
Yagi, Kiyohito
description In search of an efficient nonviral vector, polyethyleneimine (PEI)-based vectors were examined. In general, the transfection efficiency of nonviral vectors is suppressed by serum. Here we show that PEI based vectors, particularly, the chitosan hexamer-PEI vector, could perform efficient gene transfection into intestinal epithelial cells (IEC-6) in the presence of serum. The conjugation order of the two polymers with a plasmid (first, chitosan hexamer; second, PEI) was found to be an important factor in enhancing transfection efficiency.
doi_str_mv 10.1016/S1389-1723(02)80121-9
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source ScienceDirect Journals
subjects Biological and medical sciences
Biotechnology
CELL CULTURE
CHITOSAN
EPITHELIUM
Fundamental and applied biological sciences. Psychology
GENES
Genetic engineering
Genetic technics
INTESTINES
Methods. Procedures. Technologies
nonviral vector
PEI
serum-containing medium
TRANSFECTION
Vectors (cloning, transfer, expression). Insertion sequences and transposons
title Polyethyleneimine/chitosan hexamer-mediated gene transfection into intestinal epithelial cell cultured in serum-containing medium
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