Effect of static pressure on human granulocyte-macrophage stimulating factor (hGM-CSF) production by Chinese hamster ovary cells

The effect of static pressure on the production of human granulocyte-macrophage colony stimulating factor (hGM-CSF) by the Chinese hamster ovary (CHO) cell line DR1000L4N was investigated. Although constant static pressures between 0.15 and 0.9 MPa had little influence on specific growth rate, the s...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2002, Vol.94 (3), p.271-274
Main Authors: Gong, Heng, Takagi, Mutsumi, Moriyama, Tetsuhiro, Ohno, Tsuyoshi, Yoshida, Toshiomi
Format: Article
Language:English
Subjects:
Antineoplastic drugs. Cytokines
Biological and medical sciences
Biotechnology
CHO cells
Fundamental and applied biological sciences. Psychology
GM-CSF
Health. Pharmaceutical industry
Industrial applications and implications. Economical aspects
mRNA
Production of active biomolecules
protein production
static pressure
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Summary:The effect of static pressure on the production of human granulocyte-macrophage colony stimulating factor (hGM-CSF) by the Chinese hamster ovary (CHO) cell line DR1000L4N was investigated. Although constant static pressures between 0.15 and 0.9 MPa had little influence on specific growth rate, the specific production rate of hGM-CSF increased from 1.23 to 1.62 × 10 −13 mg/cell/h in proportion to static pressure. The specific rate of tPA production by the human embryo lung cell line MRC-5 also increased from 2.92 to 6.38 × 10 −12 mg/cell/h as static pressure increased. The expression level of hGM-CSF mRNA increased with the pressurization even for 4 h.
ISSN:1389-1723
1347-4421
DOI:10.1016/S1389-1723(02)80161-X