Acetylacetoin synthase as a marker enzyme for detecting the 2,3-butanediol cycle

Acetylacetoin synthase (AACSase) and acetylacetoin reductase (AACRase) are representative enzymes of the 2,3-butanediol cycle. After examining their induction conditions in various bacteria, the former was induced by acetoin and the latter by glucose. All strains carrying AACSase also had AACRase, b...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2002, Vol.93 (2), p.248-251
Main Authors: Ui, Sadaharu, Hosaka, Takeshi, Mizutani, Kimiya, Ohtsuki, Takashi, Mimura, Akio
Format: Article
Language:English
Subjects:
3-butanediol cycle
3-butanediol stereoisomer
acetylacetoin
acetylacetoin reductase
acetylacetoin synthase
BACTERIA
BIOCHEMICAL PATHWAYS
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Fundamental and applied biological sciences. Psychology
KETONES
LYASES
Mission oriented research
Physiology and metabolism
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Summary:Acetylacetoin synthase (AACSase) and acetylacetoin reductase (AACRase) are representative enzymes of the 2,3-butanediol cycle. After examining their induction conditions in various bacteria, the former was induced by acetoin and the latter by glucose. All strains carrying AACSase also had AACRase, but the reverse was not true. Therefore, AACSase indicates the existence of the cycle. Acetylacetoin (AAC) accumulation or the ratio of 2,3-butanediol isomer formed also indicated the presence of the cycle in bacteria. This cycle is present in some strains and not in others even for those belonging to the same species. The cycle was not always associated with the representative 2,3-butanediol-producing bacteria or bacterial sporogenesis as reported previously.
ISSN:1389-1723
1347-4421
DOI:10.1016/S1389-1723(02)80023-8