Isolation of enterocin SE-K4-encoding plasmid and a high enterocin SE-K4 producing strain of Enterococcus faecalis K-4

Enterococcus faecalis K-4, which produces a class IIa bacteriocin, enterocin SE-K4, carries two plasmids, pEK4S (approximately 60 kb) and pEK4L (approximately 75 kb). Plasmid-curing experiments showed that pEK4S was involved in the production of and immunity to enterocin SE-K4 in strain K-4. A deriv...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2002-04, Vol.93 (4), p.434-436
Main Authors: Doi, Katsumi, Eguchi, Tomoko, Choi, Seong-Hyun, Iwatake, Atsushi, Ohmomo, Sadahiro, Ogata, Seiya
Format: Article
Language:English
Subjects:
Antibiotics
BACTERIOCINS
Bacteriology
Biological and medical sciences
Biotechnology
enterocin-encoding plasmid
ENTEROCOCCUS
Enterococcus faecalis
Fundamental and applied biological sciences. Psychology
Health. Pharmaceutical industry
Industrial applications and implications. Economical aspects
ISOLATION
Microbiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
plasmid-encoded bacteriocin
PLASMIDS
Production of active biomolecules
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Summary:Enterococcus faecalis K-4, which produces a class IIa bacteriocin, enterocin SE-K4, carries two plasmids, pEK4S (approximately 60 kb) and pEK4L (approximately 75 kb). Plasmid-curing experiments showed that pEK4S was involved in the production of and immunity to enterocin SE-K4 in strain K-4. A derivative strain, M6, with pEK4S produced a higher amount of enterocin SE-K4 than the parental strain K-4, although its growth rate was lower than that of parental strain K-4. Phenotypic changes in strain M6 are attributed to an increase in plasmid copy number.
ISSN:1389-1723
1347-4421
DOI:10.1016/S1389-1723(02)80081-0