Difference in average survival between F344/Du and F344/N rats is not due to genetic contamination

The average survival was found to be different between substrains of rats, F344/Du and F344/N. One of the causes of this difference could have been genetic contamination by crossbreeding with other strain(s) after the divergence of substrains. These two strains were examined by PCR-SSLP (simple sequ...

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Bibliographic Details
Published in:Archives of gerontology and geriatrics 2002-02, Vol.34 (1), p.19-28
Main Authors: Tanaka, Shin, Shito, Akihito, Tamaya, Norika, Miyaishi, Osamu, Nishimura, Masahiko, Ohno, Tamio
Format: Article
Language:English
Subjects:
Average survival
F344 rats
Microsatellite (Mit) marker
Substrain
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Summary:The average survival was found to be different between substrains of rats, F344/Du and F344/N. One of the causes of this difference could have been genetic contamination by crossbreeding with other strain(s) after the divergence of substrains. These two strains were examined by PCR-SSLP (simple sequence length polymorphism) procedure using microsatellite (Mit) markers to identify the contamination. All 288 Mit markers distributed throughout the genome exhibited no size polymorphisms between these two strains. This indicated that there was no genetic contamination between these two strains. The phenotypic difference in average survival, thus, is not due to genetic contamination.
ISSN:0167-4943
1872-6976
DOI:10.1016/S0167-4943(01)00188-1