Cloning of human and rat ABCA5/Abca5 and detection of a human splice variant

We presently report the cloning of cDNA sequences encoding the novel rat ATP-binding cassette (ABC) transporter Abca5 and the orthologous human transporter, recently designated as ABCA5. Furthermore, the existence of a novel non-translated exon of the ABCA5 gene, previously assigned to an ABCA gene...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2003-01, Vol.300 (2), p.343-350
Main Authors: Petry, Frauke, Kotthaus, André, Hirsch-Ernst, Karen I
Format: Article
Language:English
Subjects:
Alternative Splicing
Amino Acid Sequence
Animals
ATP-binding cassette
ATP-Binding Cassette Transporters - classification
ATP-Binding Cassette Transporters - genetics
ATP-Binding Cassette Transporters - metabolism
Base Sequence
Cell Line
Cloning, Molecular
Gene Components
Half transporter
Hepatocytes
Hepatocytes - metabolism
Human ABCA5
Humans
Male
Molecular Sequence Data
Protein Structure, Tertiary
Rat Abca5
Rats
Rats, Wistar
RNA, Messenger - biosynthesis
Sequence Alignment
Splice variant
Tissue Distribution
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We presently report the cloning of cDNA sequences encoding the novel rat ATP-binding cassette (ABC) transporter Abca5 and the orthologous human transporter, recently designated as ABCA5. Furthermore, the existence of a novel non-translated exon of the ABCA5 gene, previously assigned to an ABCA gene cluster in the chromosomal region 17q24.2-3, is demonstrated. Abca5 and ABCA5 cDNAs are predicted to give rise to proteins of 1642 amino acids which exhibit the typical domain arrangement of ABC full transporters and share 90% identity within the amino acid sequences. A cDNA representing an ABCA5 mRNA splice variant was cloned which would result in a truncated protein equivalent to an ABC half transporter. Northern blot analyses revealed expression of ABCA5 or Abca5 mRNA in several tissues, but particularly high Abca5 mRNA expression was observed in rat testis. Up-regulation of Abca5 mRNA expression during culture of primary rat hepatocytes suggests that hepatocyte cultures should provide a basis for investigation of Abca5 gene regulation and elucidation of Abca5 function.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(02)02827-9