Effect of media supplements and culture conditions on inulinase production by an actinomycete strain

Streptomyces sp. GNDU 1 produced high levels of extra-cellular inulinase (0.552 IU/ml) after 24 h at pH 7.5, temperature 46 °C in the presence of 1% inulin. The optimum temperature and pH for enzyme activity were 60 °C and 5.5 respectively. Yeast extract as a nitrogen source was found to be most sui...

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Bibliographic Details
Published in:Bioresource technology 2003-05, Vol.87 (3), p.359-362
Main Authors: Gill, Prabhjot Kaur, Sharma, Arun Dev, Harchand, Rajesh Kumari, Singh, Prabhjeet
Format: Article
Language:English
Subjects:
Biological and medical sciences
Culture Media
Fructose
Fundamental and applied biological sciences. Psychology
Glycoside Hydrolases - biosynthesis
Hydrogen-Ion Concentration
Industry
Inulinase
Streptomyces - enzymology
Streptomyces sp
Temperature
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Summary:Streptomyces sp. GNDU 1 produced high levels of extra-cellular inulinase (0.552 IU/ml) after 24 h at pH 7.5, temperature 46 °C in the presence of 1% inulin. The optimum temperature and pH for enzyme activity were 60 °C and 5.5 respectively. Yeast extract as a nitrogen source was found to be most suitable one for inulinase production whereas ammonium ion was inhibitory to the enzymatic production. All these conditions make Streptomyces sp. GNDU 1, a potential candidate for industrial enzymatic production of fructose from inulin.
ISSN:0960-8524
1873-2976
DOI:10.1016/S0960-8524(02)00262-6