Establishment of Two Continuous T-Cell Strains from a Single Plaque of a Patient with Mycosis Fungoides

From a plaque biopsy of a patient with mycosis fungoides, two different continuous cell lines were established by including both IL-2 and IL-4 in the culture medium. Both continuous cell lines appeared with characteristic chromosome markers after approximately 40 cell population doublings. The initi...

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Bibliographic Details
Published in:In Vitro Cellular & Developmental Biology - Animal 1992-03, Vol.28A (3), p.161-167
Main Authors: Kaltoft, Keld, Susanne Bisballe, Dyrberg, Thomas, Boel, Esper, Poul Baad Rasmussen, Thestrup-Pedersen, Kristian
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Animal cells
Biological and medical sciences
Biopsies
Biotechnology
Cell Division
Cell growth
Cell lines
Chromosome Aberrations
Chromosomes
Culture Media
Cultured cells
Establishment of new cell lines, improvement of cultural methods, mass cultures
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Humans
Interleukin-2
Interleukin-4
Karyotype
Karyotyping
Male
Methods. Procedures. Technologies
Mycosis fungoides
Mycosis Fungoides - immunology
Mycosis Fungoides - pathology
Receptors, Antigen, T-Cell
Skin
Skin Neoplasms - immunology
Skin Neoplasms - pathology
T cell antigen receptors
T lymphocytes
T-Lymphocytes - cytology
T-Lymphocytes - immunology
Tumor Cells, Cultured
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Summary:From a plaque biopsy of a patient with mycosis fungoides, two different continuous cell lines were established by including both IL-2 and IL-4 in the culture medium. Both continuous cell lines appeared with characteristic chromosome markers after approximately 40 cell population doublings. The initial karyotype recognized in T cells from the skin biopsy was 46,XY and the karyotypes of the continuous cell strains were 46,XY, -18, + i(18q) and another with multiple chromosome aberrations as described in Sezary T-cell leukemia. Phenotyping with monoclonal antibodies and T-cell receptor analysis indicates that the latter cell strain represents a minority of T-cells in the plaque. Due to its many chromosomal aberrations it probably represents the malignant cell, which may be a central cell in the immune stimulation taking place in the skin.
ISSN:0883-8364
2327-431X
1543-706X
DOI:10.1007/BF02631086