Establishment of Two Continuous T-Cell Strains from a Single Plaque of a Patient with Mycosis Fungoides

• Published in: In Vitro Cellular & Developmental Biology - Animal 1992-03, Vol.28A (3), p.161-167
• Main Authors: Kaltoft, Keld, Susanne Bisballe, Dyrberg, Thomas, Boel, Esper, Poul Baad Rasmussen, Thestrup-Pedersen, Kristian
• Format: Article
• Language: English
• Subjects: Aged; Aged, 80 and over; Animal cells; Biological and medical sciences; Biopsies; Biotechnology; Cell Division; Cell growth; Cell lines; Chromosome Aberrations; Chromosomes; Culture Media; Cultured cells; Establishment of new cell lines, improvement of cultural methods, mass cultures; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; Humans; Interleukin-2; Interleukin-4; Karyotype; Karyotyping; Male; Methods. Procedures. Technologies; Mycosis fungoides; Mycosis Fungoides - immunology; Mycosis Fungoides - pathology; Receptors, Antigen, T-Cell; Skin; Skin Neoplasms - immunology; Skin Neoplasms - pathology; T cell antigen receptors; T lymphocytes; T-Lymphocytes - cytology; T-Lymphocytes - immunology; Tumor Cells, Cultured
• ISSN: 0883-8364; 2327-431X; 1543-706X
• DOI: 10.1007/BF02631086

From a plaque biopsy of a patient with mycosis fungoides, two different continuous cell lines were established by including both IL-2 and IL-4 in the culture medium. Both continuous cell lines appeared with characteristic chromosome markers after approximately 40 cell population doublings. The initial karyotype recognized in T cells from the skin biopsy was 46,XY and the karyotypes of the continuous cell strains were 46,XY, -18, + i(18q) and another with multiple chromosome aberrations as described in Sezary T-cell leukemia. Phenotyping with monoclonal antibodies and T-cell receptor analysis indicates that the latter cell strain represents a minority of T-cells in the plaque. Due to its many chromosomal aberrations it probably represents the malignant cell, which may be a central cell in the immune stimulation taking place in the skin.