Intravenous injection of naked plasmid DNA encoding hepatitis B virus (HBV) produces HBV and induces humoral immune response in mice

Hepatitis B virus (HBV) infection is highly specific to primates. To cross the species barrier, we injected naked plasmid DNA containing a 1.5-fold-overlength genome of HBV into immunocompetent mice via the tail vein with acute circulatory overload. The injection resulted in production of viral tran...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2003-01, Vol.300 (3), p.784-788
Main Authors: Suzuki, Takahiro, Takehara, Tetsuo, Ohkawa, Kazuyoshi, Ishida, Hisashi, Jinushi, Masahisa, Miyagi, Takuya, Sasaki, Yutaka, Hayashi, Norio
Format: Article
Language:English
Subjects:
Animal model
Animals
Antibody Formation - immunology
Disease Models, Animal
Female
Gene expression
Gene transfer
Hepatitis B - immunology
Hepatitis B - virology
Hepatitis B Antibodies - blood
Hepatitis B Core Antigens - biosynthesis
Hepatitis B Surface Antigens - blood
Hepatitis B Surface Antigens - immunology
Hepatitis B virus
Hepatitis B virus - genetics
Hepatitis B virus - growth & development
Hepatitis B virus - immunology
Hydrodynamics-based gene delivery
Injections, Intravenous
Liver - metabolism
Liver - virology
Mice
Mice, Inbred BALB C
Mouse
Naked plasmid DNA
Plasmids - administration & dosage
Plasmids - genetics
Replication
RNA, Viral - metabolism
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Summary:Hepatitis B virus (HBV) infection is highly specific to primates. To cross the species barrier, we injected naked plasmid DNA containing a 1.5-fold-overlength genome of HBV into immunocompetent mice via the tail vein with acute circulatory overload. The injection resulted in production of viral transcripts specifically in the liver and expression of hepatitis B core protein in approximately 3% of the hepatocytes. HBV was secreted into the blood, evidenced by the presence of DNase I-resistant HBV sequence in fractionated serum at a density of 1.21 g/ml. The HBV DNA positivity in the serum persisted for 1 week. Most mice became positive for hepatitis B surface antigen for 2 weeks and later seropositive for anti-hepatitis B surface antibody. This simple and efficient HBV replication system in mice could be useful for investigating whether viral mutations as well as host genetic background may affect viral replication and induction of immune response.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(02)02889-9