MTO: the second member of a Drosophila dual copper-thionein system

Drosophila MTO metal binding features were analyzed for comparison with MTN, the paralogous Drosophila metallothionein, and to classify MTO as either zinc- or copper-thionein. This was achieved by a combination of in vivo, in vitro and in silico methodologies. All the results unambiguously classifie...

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Bibliographic Details
Published in:FEBS letters 2003-01, Vol.533 (1), p.72-78
Main Authors: Domenech, J., Palacios, O., Villarreal, L., González-Duarte, P., Capdevila, M., Atrian, S.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cd(II) binding
CD, circular dichroism
Cloning, Molecular
Copper - chemistry
Cu(I) binding
DNA, Complementary - genetics
Drosophila
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Drosophila metallothionein
Drosophila Proteins - chemistry
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
ESI-MS, electrospray ionization mass spectrometry
ICP-AES, inductively coupled plasma atomic emission spectroscopy
Metallothionein - chemistry
Metallothionein - genetics
Metallothionein - metabolism
Molecular evolution
Molecular Sequence Data
Molecular Structure
MT, metallothionein
Phylogeny
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Recombinant synthesis
Sequence Homology, Amino Acid
UV-vis, electronic absorption
Zinc - chemistry
Zn(II) binding
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Summary:Drosophila MTO metal binding features were analyzed for comparison with MTN, the paralogous Drosophila metallothionein, and to classify MTO as either zinc- or copper-thionein. This was achieved by a combination of in vivo, in vitro and in silico methodologies. All the results unambiguously classified MTO as a second Drosophila copper-thionein, putting Drosophila forward as the only metazoan in which any zinc-thionein has still to be reported. Interestingly, experimental data only showed minor differences in the coordinative behavior of both MTs, but provided a characteristic spectroscopic fingerprint, revealing the possible binding of chloride anions in certain metal-MTO aggregates.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(02)03754-7