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Cloning and sequence analysis of cDNA for a neuronal cell membrane antigen, HPC-1
A monoclonal antibody (mAb), HPC-1, labels the plasma membrane of the amacrine cell soma and inner plexiform layer in rat retina and other central neurons. HPC-1 antigen recognizes several proteins of about 35 kDa. In this study, an HPC-1 positive cDNA, HPC-113, was isolated from a lambda gt11 cDNA...
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Published in: | The Journal of biological chemistry 1992-05, Vol.267 (15), p.10613-10619 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | A monoclonal antibody (mAb), HPC-1, labels the plasma membrane of the amacrine cell soma and inner plexiform layer in rat
retina and other central neurons. HPC-1 antigen recognizes several proteins of about 35 kDa. In this study, an HPC-1 positive
cDNA, HPC-113, was isolated from a lambda gt11 cDNA library of the rat hippocampus. HPC-113 had the 894-base pair nucleotide
sequence in an open reading frame and the calculated molecular mass of the deduced amino acid sequence (298 residues) was
33,989 Da, implying that HPC-113 contains almost the full-length coding region of HPC-1 antigen is an integrated membrane
protein revealing the characteristic alpha-helical structure with periodical heptad repeats usually seen in proteins with
coiled-coil structures. Although the entire amino acid sequence did not show significant homology to any proteins so far known,
a few local sequences in the possible extracellular domain of the HPC-1 antigen molecule had notable homology to some partial
sequences in the laminin B1 chain. These sequences of laminin are included in the portion which has neurite outgrowth and/or
survival promoting activity. The HPC-1 gene was transcribed in nerve tissues much more predominantly than in non-neuronal
tissues. Thus, HPC-1 antigen(s) was confined to be a newly identified neuronal cell membrane protein(s) localized in a subpopulation
of neurons. |
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ISSN: | 0021-9258 1083-351X |
DOI: | 10.1016/s0021-9258(19)50061-8 |