Identification and quantification of metallothionein isoforms and superoxide dismutase in spiked liver extracts using HPLC-ESI-MS offline coupling and HPLC-ICP-MS online coupling

A two-dimensional chromatographic method for the characterization of metallothionein isoforms (MT) and superoxide dismutase (SOD) in spiked liver extracts was developed for the optimization of extraction procedures from liver samples. Element-specific detection (ICP-MS) and molecule-specific detecti...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2003-01, Vol.375 (1), p.145-156
Main Authors: Nischwitz, V, Michalke, B, Kettrup, A
Format: Article
Language:English
Subjects:
Animals
Calibration
Chromatography, High Pressure Liquid
In Vitro Techniques
Liver - enzymology
Liver - metabolism
Mass Spectrometry - instrumentation
Mass Spectrometry - methods
Metallothionein - isolation & purification
Protein Isoforms - isolation & purification
Superoxide Dismutase - isolation & purification
Swine
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Summary:A two-dimensional chromatographic method for the characterization of metallothionein isoforms (MT) and superoxide dismutase (SOD) in spiked liver extracts was developed for the optimization of extraction procedures from liver samples. Element-specific detection (ICP-MS) and molecule-specific detection (ESI-MS) were applied for maximum species information. A special focus was laid on the quantitative data evaluation (species stoichiometry, calibration with and without matrix, recovery), which is neglected in most MT/SOD publications with hyphenated techniques. Linearity, precision (residual standard deviation of calibration curves
ISSN:1618-2642
DOI:10.1007/s00216-002-1594-2