Transcriptional profiling of gene expression patterns during sphingosine 1-phosphate-induced mesangial cell proliferation

Sphingosine 1-phosphate (S1P) is known to regulate cell proliferation, apoptosis, and motility. Recently, we have reported that S1P and its analogue dihydro-S1P (DHS1P) promote proliferation of rat cultured mesangial cells. To investigate the signaling mechanisms underlying S1P- and DHS1P-induced me...

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Published in:Biochemical and biophysical research communications 2003-01, Vol.300 (2), p.577-584
Main Authors: Katsuma, Susumu, Hada, Yuko, Shiojima, Satoshi, Hirasawa, Akira, Tanoue, Akito, Takagaki, Kazuchika, Ohgi, Tadaaki, Yano, Junichi, Tsujimoto, Gozoh
Format: Article
Language:English
Subjects:
Animals
Calcium - analysis
Calcium mobilization
cDNA microarray
Cell Division
Gene expression
Gene Expression Profiling
Glomerular Mesangium - chemistry
Glomerular Mesangium - drug effects
Glomerular Mesangium - metabolism
Kinetics
Lysophospholipids
Mesangial cells
Oligonucleotide Array Sequence Analysis
Rats
Sphingosine - analogs & derivatives
Sphingosine - pharmacology
Sphingosine 1-phosphate
Transcription, Genetic
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Summary:Sphingosine 1-phosphate (S1P) is known to regulate cell proliferation, apoptosis, and motility. Recently, we have reported that S1P and its analogue dihydro-S1P (DHS1P) promote proliferation of rat cultured mesangial cells. To investigate the signaling mechanisms underlying S1P- and DHS1P-induced mesangial cell proliferation, we performed cDNA microarray analysis of gene expression during mesangial cell proliferation. In terms of the overall pattern, gene expression waves induced by S1P and DHS1P were similar to those induced by a potent mesangial mitogen platelet-derived growth factor (PDGF), whereas we found several genes, such as two growth factors, connective tissue growth factor (CTGF) and heparin-binding EGF-like growth factor (HB-EGF), which were induced by the sphingolipids, but not by PDGF. Cluster analysis also identified calcium-dependent molecules highly expressed in DHS1P-stimulated cells compared to S1P-stimulated cells. Calcium mobilization analysis showed that DHS1P had higher magnitudes of intracellular calcium mobilization than S1P, suggesting that S1P and DHS1P differentially regulate intracellular calcium mobilization, possibly leading to different gene expression in mesangial cells. The large-scale monitoring of gene expression performed here allows us to identify S1P-induced transcriptional properties during mesangial cell proliferation.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(02)02850-4