Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and B and Flagellin

Recombinant (r) outer surface proteins (Osp) A and B, flagellin, and an immunogenic region of flagellin (41-G) from Borrelia burgdorferi were evaluated using immunoblot and ELISA for usefulness as substrates in diagnostic testing for Lyme disease. Antibodies to rOspA, rOspB, and r-flagellin were det...

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Bibliographic Details
Published in:The Journal of infectious diseases 1992-06, Vol.165 (6), p.1127-1132
Main Authors: Fikrig, Erol, Huguenel, Edward D., Berland, Robert, Rahn, Daniel W., Hardin, John A., Flavell, Richard A.
Format: Article
Language:English
Subjects:
Antibodies
Antibodies, Bacterial - blood
Antibodies, Monoclonal - immunology
Antigens
Antigens, Bacterial - immunology
Bacterial diseases
Bacterial Outer Membrane Proteins - immunology
Biological and medical sciences
Borrelia burgdorferi
Borrelia burgdorferi Group - immunology
Borrelia infections
Concise Communications
Enzyme linked immunosorbent assay
Erythema
Flagellin - immunology
Human bacterial diseases
Humans
Immunoblotting
Infectious diseases
Lyme disease
Lyme Disease - diagnosis
Medical sciences
Reactivity
Recombinant antigens
Recombinant Fusion Proteins - immunology
Recombinant Proteins - immunology
Spirochaetales
Syphilis
Tropical bacterial diseases
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Summary:Recombinant (r) outer surface proteins (Osp) A and B, flagellin, and an immunogenic region of flagellin (41-G) from Borrelia burgdorferi were evaluated using immunoblot and ELISA for usefulness as substrates in diagnostic testing for Lyme disease. Antibodies to rOspA, rOspB, and r-flagellin were detected by immunoblot and ELISA using the recombinant proteins. Patients with late disease responded to rOspA, rOspB, and r-flagellin, or only to r-flagellin, whereas patients with early disease showed no response or responded only to r-flagellin. Patients who developed antibodies to r-flagellin also responded to 41-G. The data suggest that recombinant B. burgdorferi antigens can serve as substrates for immunoblot and ELISA, which may be helpful in diagnosing Lyme disease.
ISSN:0022-1899
1537-6613