Loading…
Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and B and Flagellin
Recombinant (r) outer surface proteins (Osp) A and B, flagellin, and an immunogenic region of flagellin (41-G) from Borrelia burgdorferi were evaluated using immunoblot and ELISA for usefulness as substrates in diagnostic testing for Lyme disease. Antibodies to rOspA, rOspB, and r-flagellin were det...
Saved in:
| Published in: | The Journal of infectious diseases 1992-06, Vol.165 (6), p.1127-1132 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | |
|---|---|
| cites | |
| container_end_page | 1132 |
| container_issue | 6 |
| container_start_page | 1127 |
| container_title | The Journal of infectious diseases |
| container_volume | 165 |
| creator | Fikrig, Erol Huguenel, Edward D. Berland, Robert Rahn, Daniel W. Hardin, John A. Flavell, Richard A. |
| description | Recombinant (r) outer surface proteins (Osp) A and B, flagellin, and an immunogenic region of flagellin (41-G) from Borrelia burgdorferi were evaluated using immunoblot and ELISA for usefulness as substrates in diagnostic testing for Lyme disease. Antibodies to rOspA, rOspB, and r-flagellin were detected by immunoblot and ELISA using the recombinant proteins. Patients with late disease responded to rOspA, rOspB, and r-flagellin, or only to r-flagellin, whereas patients with early disease showed no response or responded only to r-flagellin. Patients who developed antibodies to r-flagellin also responded to 41-G. The data suggest that recombinant B. burgdorferi antigens can serve as substrates for immunoblot and ELISA, which may be helpful in diagnosing Lyme disease. |
| format | article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_72949087</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>30112202</jstor_id><sourcerecordid>30112202</sourcerecordid><originalsourceid>FETCH-LOGICAL-j203t-634c094a464ae937ff71bc58584f7d2abb428882c6b43821639ea78c0ee95a623</originalsourceid><addsrcrecordid>eNqFkE1Lw0AQhoMotX78BGEP4i2wX9mPY61WhYJi9Vwm20nZkuzW3eTgvzdoEW_OYQbmfXgY5qiYskroUikmjosppZyXzFh7WpzlvKOUSqH0pJiwyoixpkW9whTbuPWO3HnYhph9JrEhy88Ox01GyEiG7MOWvKKLXe0DhJ48Dz0mshpSAw7JS4o9-pDJjEDYkNvvvmhhi23rw0Vx0kCb8fIwz4v3xf3b_LFcPj88zWfLcsep6EslpKNWglQS0ArdNJrVrjKVkY3ecKhryY0x3KlaCsOZEhZBG0cRbQWKi_Pi5se7T_FjwNyvO5_deAIEjENea26lpUb_C45qppSuRvDqAA51h5v1PvkO0uf68L0xvz7kkB20TYLgfP7FKmm14n80u9zH9BsLyhjnlIsvAqKBRw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16316675</pqid></control><display><type>article</type><title>Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and B and Flagellin</title><source>JSTOR Archival Journals and Primary Sources Collection</source><source>Oxford University Press Archive</source><creator>Fikrig, Erol ; Huguenel, Edward D. ; Berland, Robert ; Rahn, Daniel W. ; Hardin, John A. ; Flavell, Richard A.</creator><creatorcontrib>Fikrig, Erol ; Huguenel, Edward D. ; Berland, Robert ; Rahn, Daniel W. ; Hardin, John A. ; Flavell, Richard A.</creatorcontrib><description>Recombinant (r) outer surface proteins (Osp) A and B, flagellin, and an immunogenic region of flagellin (41-G) from Borrelia burgdorferi were evaluated using immunoblot and ELISA for usefulness as substrates in diagnostic testing for Lyme disease. Antibodies to rOspA, rOspB, and r-flagellin were detected by immunoblot and ELISA using the recombinant proteins. Patients with late disease responded to rOspA, rOspB, and r-flagellin, or only to r-flagellin, whereas patients with early disease showed no response or responded only to r-flagellin. Patients who developed antibodies to r-flagellin also responded to 41-G. The data suggest that recombinant B. burgdorferi antigens can serve as substrates for immunoblot and ELISA, which may be helpful in diagnosing Lyme disease.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>PMID: 1583333</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: University of Chicago Press</publisher><subject>Antibodies ; Antibodies, Bacterial - blood ; Antibodies, Monoclonal - immunology ; Antigens ; Antigens, Bacterial - immunology ; Bacterial diseases ; Bacterial Outer Membrane Proteins - immunology ; Biological and medical sciences ; Borrelia burgdorferi ; Borrelia burgdorferi Group - immunology ; Borrelia infections ; Concise Communications ; Enzyme linked immunosorbent assay ; Erythema ; Flagellin - immunology ; Human bacterial diseases ; Humans ; Immunoblotting ; Infectious diseases ; Lyme disease ; Lyme Disease - diagnosis ; Medical sciences ; Reactivity ; Recombinant antigens ; Recombinant Fusion Proteins - immunology ; Recombinant Proteins - immunology ; Spirochaetales ; Syphilis ; Tropical bacterial diseases</subject><ispartof>The Journal of infectious diseases, 1992-06, Vol.165 (6), p.1127-1132</ispartof><rights>Copyright 1992 The University of Chicago</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30112202$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30112202$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,58213,58446</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5497625$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1583333$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fikrig, Erol</creatorcontrib><creatorcontrib>Huguenel, Edward D.</creatorcontrib><creatorcontrib>Berland, Robert</creatorcontrib><creatorcontrib>Rahn, Daniel W.</creatorcontrib><creatorcontrib>Hardin, John A.</creatorcontrib><creatorcontrib>Flavell, Richard A.</creatorcontrib><title>Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and B and Flagellin</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>Recombinant (r) outer surface proteins (Osp) A and B, flagellin, and an immunogenic region of flagellin (41-G) from Borrelia burgdorferi were evaluated using immunoblot and ELISA for usefulness as substrates in diagnostic testing for Lyme disease. Antibodies to rOspA, rOspB, and r-flagellin were detected by immunoblot and ELISA using the recombinant proteins. Patients with late disease responded to rOspA, rOspB, and r-flagellin, or only to r-flagellin, whereas patients with early disease showed no response or responded only to r-flagellin. Patients who developed antibodies to r-flagellin also responded to 41-G. The data suggest that recombinant B. burgdorferi antigens can serve as substrates for immunoblot and ELISA, which may be helpful in diagnosing Lyme disease.</description><subject>Antibodies</subject><subject>Antibodies, Bacterial - blood</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens</subject><subject>Antigens, Bacterial - immunology</subject><subject>Bacterial diseases</subject><subject>Bacterial Outer Membrane Proteins - immunology</subject><subject>Biological and medical sciences</subject><subject>Borrelia burgdorferi</subject><subject>Borrelia burgdorferi Group - immunology</subject><subject>Borrelia infections</subject><subject>Concise Communications</subject><subject>Enzyme linked immunosorbent assay</subject><subject>Erythema</subject><subject>Flagellin - immunology</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Infectious diseases</subject><subject>Lyme disease</subject><subject>Lyme Disease - diagnosis</subject><subject>Medical sciences</subject><subject>Reactivity</subject><subject>Recombinant antigens</subject><subject>Recombinant Fusion Proteins - immunology</subject><subject>Recombinant Proteins - immunology</subject><subject>Spirochaetales</subject><subject>Syphilis</subject><subject>Tropical bacterial diseases</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNqFkE1Lw0AQhoMotX78BGEP4i2wX9mPY61WhYJi9Vwm20nZkuzW3eTgvzdoEW_OYQbmfXgY5qiYskroUikmjosppZyXzFh7WpzlvKOUSqH0pJiwyoixpkW9whTbuPWO3HnYhph9JrEhy88Ox01GyEiG7MOWvKKLXe0DhJ48Dz0mshpSAw7JS4o9-pDJjEDYkNvvvmhhi23rw0Vx0kCb8fIwz4v3xf3b_LFcPj88zWfLcsep6EslpKNWglQS0ArdNJrVrjKVkY3ecKhryY0x3KlaCsOZEhZBG0cRbQWKi_Pi5se7T_FjwNyvO5_deAIEjENea26lpUb_C45qppSuRvDqAA51h5v1PvkO0uf68L0xvz7kkB20TYLgfP7FKmm14n80u9zH9BsLyhjnlIsvAqKBRw</recordid><startdate>199206</startdate><enddate>199206</enddate><creator>Fikrig, Erol</creator><creator>Huguenel, Edward D.</creator><creator>Berland, Robert</creator><creator>Rahn, Daniel W.</creator><creator>Hardin, John A.</creator><creator>Flavell, Richard A.</creator><general>University of Chicago Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>199206</creationdate><title>Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and B and Flagellin</title><author>Fikrig, Erol ; Huguenel, Edward D. ; Berland, Robert ; Rahn, Daniel W. ; Hardin, John A. ; Flavell, Richard A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j203t-634c094a464ae937ff71bc58584f7d2abb428882c6b43821639ea78c0ee95a623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Antibodies</topic><topic>Antibodies, Bacterial - blood</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigens</topic><topic>Antigens, Bacterial - immunology</topic><topic>Bacterial diseases</topic><topic>Bacterial Outer Membrane Proteins - immunology</topic><topic>Biological and medical sciences</topic><topic>Borrelia burgdorferi</topic><topic>Borrelia burgdorferi Group - immunology</topic><topic>Borrelia infections</topic><topic>Concise Communications</topic><topic>Enzyme linked immunosorbent assay</topic><topic>Erythema</topic><topic>Flagellin - immunology</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Infectious diseases</topic><topic>Lyme disease</topic><topic>Lyme Disease - diagnosis</topic><topic>Medical sciences</topic><topic>Reactivity</topic><topic>Recombinant antigens</topic><topic>Recombinant Fusion Proteins - immunology</topic><topic>Recombinant Proteins - immunology</topic><topic>Spirochaetales</topic><topic>Syphilis</topic><topic>Tropical bacterial diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fikrig, Erol</creatorcontrib><creatorcontrib>Huguenel, Edward D.</creatorcontrib><creatorcontrib>Berland, Robert</creatorcontrib><creatorcontrib>Rahn, Daniel W.</creatorcontrib><creatorcontrib>Hardin, John A.</creatorcontrib><creatorcontrib>Flavell, Richard A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fikrig, Erol</au><au>Huguenel, Edward D.</au><au>Berland, Robert</au><au>Rahn, Daniel W.</au><au>Hardin, John A.</au><au>Flavell, Richard A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and B and Flagellin</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1992-06</date><risdate>1992</risdate><volume>165</volume><issue>6</issue><spage>1127</spage><epage>1132</epage><pages>1127-1132</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Recombinant (r) outer surface proteins (Osp) A and B, flagellin, and an immunogenic region of flagellin (41-G) from Borrelia burgdorferi were evaluated using immunoblot and ELISA for usefulness as substrates in diagnostic testing for Lyme disease. Antibodies to rOspA, rOspB, and r-flagellin were detected by immunoblot and ELISA using the recombinant proteins. Patients with late disease responded to rOspA, rOspB, and r-flagellin, or only to r-flagellin, whereas patients with early disease showed no response or responded only to r-flagellin. Patients who developed antibodies to r-flagellin also responded to 41-G. The data suggest that recombinant B. burgdorferi antigens can serve as substrates for immunoblot and ELISA, which may be helpful in diagnosing Lyme disease.</abstract><cop>Chicago, IL</cop><pub>University of Chicago Press</pub><pmid>1583333</pmid><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1899 |
| ispartof | The Journal of infectious diseases, 1992-06, Vol.165 (6), p.1127-1132 |
| issn | 0022-1899 1537-6613 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72949087 |
| source | JSTOR Archival Journals and Primary Sources Collection; Oxford University Press Archive |
| subjects | Antibodies Antibodies, Bacterial - blood Antibodies, Monoclonal - immunology Antigens Antigens, Bacterial - immunology Bacterial diseases Bacterial Outer Membrane Proteins - immunology Biological and medical sciences Borrelia burgdorferi Borrelia burgdorferi Group - immunology Borrelia infections Concise Communications Enzyme linked immunosorbent assay Erythema Flagellin - immunology Human bacterial diseases Humans Immunoblotting Infectious diseases Lyme disease Lyme Disease - diagnosis Medical sciences Reactivity Recombinant antigens Recombinant Fusion Proteins - immunology Recombinant Proteins - immunology Spirochaetales Syphilis Tropical bacterial diseases |
| title | Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and B and Flagellin |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T12%3A43%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Serologic%20Diagnosis%20of%20Lyme%20Disease%20using%20Recombinant%20Outer%20Surface%20Proteins%20A%20and%20B%20and%20Flagellin&rft.jtitle=The%20Journal%20of%20infectious%20diseases&rft.au=Fikrig,%20Erol&rft.date=1992-06&rft.volume=165&rft.issue=6&rft.spage=1127&rft.epage=1132&rft.pages=1127-1132&rft.issn=0022-1899&rft.eissn=1537-6613&rft.coden=JIDIAQ&rft_id=info:doi/&rft_dat=%3Cjstor_proqu%3E30112202%3C/jstor_proqu%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-j203t-634c094a464ae937ff71bc58584f7d2abb428882c6b43821639ea78c0ee95a623%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=16316675&rft_id=info:pmid/1583333&rft_jstor_id=30112202&rfr_iscdi=true |