A comparative study of protein immobilization techniques for optical immunosensors

This paper presents the results of a study of a number of antibody immobilization techniques for application to optical immunosensors. In particular, well-known methods such as covalent binding and physical adsorption have been extended to the Langmuir-Blodgett method in an attempt to improve the de...

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Bibliographic Details
Published in:Biosensors & bioelectronics 1992, Vol.7 (3), p.207-214
Main Authors: Ahluwalia, A., De Rossi, D., Ristori, C., Schirone, A., Serra, G.
Format: Article
Language:English
Subjects:
Adsorption
Antibodies
Avidin - chemistry
Biological and medical sciences
Biosensing Techniques
Biotechnology
Enzyme-Linked Immunosorbent Assay
Fluorescence
Fundamental and applied biological sciences. Psychology
Immobilization of enzymes and other molecules
Immobilization techniques
Immunoglobulin G - immunology
immunosensors
Langmuir-Blodgett films
Methods. Procedures. Technologies
Models, Biological
Optics and Photonics
Prolactin - immunology
protein immobilization
Proteins - chemistry
Serum Albumin, Bovine - chemistry
Silanes
Surface Properties
total internal reflection fluorescence
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Summary:This paper presents the results of a study of a number of antibody immobilization techniques for application to optical immunosensors. In particular, well-known methods such as covalent binding and physical adsorption have been extended to the Langmuir-Blodgett method in an attempt to improve the density and possibly the uniformity of orientation of monoclonal antibodies on an optical surface. The surface density of active immobilized antibodies was determined from enzyme immunoassay and their thickness and refractive index were deduced from ellipsometry. It is shown that, although high surface densities (500ng/cm 2) of antibody can be obtained, the major obstacle to the detection of low concentrations of antigens or haptens is the non-specific binding of foreign molecules to the sensing surface.
ISSN:0956-5663
1873-4235
DOI:10.1016/0956-5663(92)87017-J