Sephadex and sephadex ion-exchange filtration improves the quality and freezability of low-grade buffalo semen ejaculates

The effect of sephadex and sephadex ion-exchange filtration on the improvement in quality and freezability of low-grade buffalo semen ejaculates was assessed. Two types of filtration columns were used: one containing only sephadex G-10 (FS) and the other sephadex G-10 along with ion-exchangers (diet...

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Bibliographic Details
Published in:Theriogenology 2003-03, Vol.59 (5), p.1189-1202
Main Authors: Ahmad, Z, Anzar, M, Shahab, M, Ahmad, N, Andrabi, S.M.H
Format: Article
Language:English
Subjects:
Acrosome - physiology
Animals
Buffalo spermatozoa
Buffaloes - physiology
Cell Membrane - physiology
Chromatography, Gel - veterinary
Chromatography, Ion Exchange - veterinary
Cryopreservation
Cryopreservation - methods
Cryopreservation - veterinary
Male
Semen Preservation - methods
Semen Preservation - veterinary
Sephadex ion-exchange
Sperm Motility - physiology
Spermatozoa - physiology
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Summary:The effect of sephadex and sephadex ion-exchange filtration on the improvement in quality and freezability of low-grade buffalo semen ejaculates was assessed. Two types of filtration columns were used: one containing only sephadex G-10 (FS) and the other sephadex G-10 along with ion-exchangers (diethyl amino ethane-52 (DEAE-52) cellulose and carboxy methyl-52 (CM-52) cellulose; FS+IE). Unfiltered samples served as controls. Semen ejaculates extended in Tris–citric acid (1:4) ( n=16; initial motility 40–50%) were filtered at the rate of 1.5 ml/min under negative pressure at room temperature (28–30 °C). The mean recovery rate (%) of motile spermatozoa in the FS (85.9±1.51) and FS+IE (77.10±2.28) filtrates did not differ significantly. Percentages of sperm motility, normal acrosomes, and intact plasma membranes were highest ( P
ISSN:0093-691X
1879-3231
DOI:10.1016/S0093-691X(02)01159-7