Hepatitis E Virus: cDNA Cloning and Expression

Viral hepatitis E is endemic, frequently provoking epidemic outbreaks in many developing countries. We have attempted to clone the viral genome and to develop an antibody assay system. A lambda gt11 cDNA library was constructed from the bile juice containing putative causative viruses and was immuno...

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Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 1992, Vol.36(1), pp.67-79
Main Authors: Uchida, Toshikazu, Suzuki, Koyu, Hayashi, Nakanobu, Iida, Fusae, Hara, Tomijiro, Oo, San San, Wang, Chung-kwe, Shikata, Toshio, Ichikawa, Munetaka, Rikihisa, Tetsuji, Mizuno, Kyosuke, Win, Khin Maung
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies
Base Sequence
Bile - chemistry
Biological and medical sciences
Cloning, Molecular
DNA, Viral - genetics
DNA, Viral - isolation & purification
Fundamental and applied biological sciences. Psychology
Gene Expression - genetics
Genetics
Hepatitis Antibodies - blood
Hepatitis E virus
Hepatitis E virus - genetics
Hepatitis E virus - immunology
Humans
Immunoassay
Macaca mulatta
Microbiology
Molecular Sequence Data
Polymerase Chain Reaction
Recombinant Fusion Proteins - immunology
Virology
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Summary:Viral hepatitis E is endemic, frequently provoking epidemic outbreaks in many developing countries. We have attempted to clone the viral genome and to develop an antibody assay system. A lambda gt11 cDNA library was constructed from the bile juice containing putative causative viruses and was immunoscreened by the antisera obtained from patients and monkeys infected with hepatitis E. Three virus-specific clones were isolated and were revealed to overlap one another in sequence, with 1, 459 nucleotides in total length. These clones direct the synthesis of polypeptides probably having common immunological epitope(s). Immunoplaque assay revealed the occurrence of antibodies against this epitope in the sera from experimental monkeys with the convalescent phase and from patients of Myanmar, Nepal and India. The data indicate that the cDNA fragments are useful for immunodiagnosis of hepatitis E.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1992.tb01643.x