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Simian varicella virus: characterization of virion and infected cell polypeptides and the antigenic cross-reactivity with varicella-zoster virus
Department of Microbiology and Immunology, 4301 West Markham Street, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, U.S.A. Simian varicella virus (SVV) causes a varicella-like disease in non-human primates. In this study, SVV virions were purified from SVV-infected BSC-1 c...
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| Published in: | Journal of general virology 1992-05, Vol.73 (5), p.1209-1215 |
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| container_end_page | 1215 |
| container_issue | 5 |
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| container_title | Journal of general virology |
| container_volume | 73 |
| creator | Fletcher, Thomas M., III Gray, Wayne L |
| description | Department of Microbiology and Immunology, 4301 West Markham Street, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, U.S.A.
Simian varicella virus (SVV) causes a varicella-like disease in non-human primates. In this study, SVV virions were purified from SVV-infected BSC-1 cells by zonal and differential gradient centrifugation and the virion polypeptide composition was analysed by SDS-PAGE. SVV virions had a buoyant density of 1.21 g/ml, identical to the value obtained for varicella-zoster virus (VZV) virions purified by the same method. Electron microscopy of the concentrated SVV virions revealed characteristic herpesvirus morphology. SVV virions consisted of at least 30 polypeptide species ranging from 16K to >200K. The electrophoretic profiles of radiolabelled SVV and VZV virion polypeptides were very similar. Immunoprecipitations of solubilized SVV-infected cell preparations using SVV immune sera revealed at least 18 viral polypeptides with an M r range of 12K to 142K and six glycoproteins ranging from 46K to 115K. In addition, extensive cross-reactivity between SVV and VZV proteins and glycoproteins was demonstrated by immunoprecipitation with heterologous immune sera. The high degree of antigenic relatedness between SVV and VZV provides further support for simian varicella as a model for VZV infections.
Received 14 October 1991;
accepted 24 January 1992. |
| doi_str_mv | 10.1099/0022-1317-73-5-1209 |
| format | article |
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Simian varicella virus (SVV) causes a varicella-like disease in non-human primates. In this study, SVV virions were purified from SVV-infected BSC-1 cells by zonal and differential gradient centrifugation and the virion polypeptide composition was analysed by SDS-PAGE. SVV virions had a buoyant density of 1.21 g/ml, identical to the value obtained for varicella-zoster virus (VZV) virions purified by the same method. Electron microscopy of the concentrated SVV virions revealed characteristic herpesvirus morphology. SVV virions consisted of at least 30 polypeptide species ranging from 16K to >200K. The electrophoretic profiles of radiolabelled SVV and VZV virion polypeptides were very similar. Immunoprecipitations of solubilized SVV-infected cell preparations using SVV immune sera revealed at least 18 viral polypeptides with an M r range of 12K to 142K and six glycoproteins ranging from 46K to 115K. In addition, extensive cross-reactivity between SVV and VZV proteins and glycoproteins was demonstrated by immunoprecipitation with heterologous immune sera. The high degree of antigenic relatedness between SVV and VZV provides further support for simian varicella as a model for VZV infections.
Received 14 October 1991;
accepted 24 January 1992.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-73-5-1209</identifier><identifier>PMID: 1316940</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Animals ; Biological and medical sciences ; Cercopithecus aethiops ; Cross Reactions ; Disease Models, Animal ; Erythrocebus patas ; Evaluation Studies as Topic ; Fundamental and applied biological sciences. Psychology ; Glycoproteins - chemistry ; Glycoproteins - immunology ; Hepatitis Delta Virus - chemistry ; Hepatitis Delta Virus - immunology ; Herpesvirus 3, Human - chemistry ; Herpesvirus 3, Human - immunology ; Microbiology ; Precipitin Tests ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; varicella-zoster virus ; Viral Proteins - chemistry ; Viral Proteins - immunology ; Virion - chemistry ; Virion - immunology ; Virion - isolation & purification ; Virology</subject><ispartof>Journal of general virology, 1992-05, Vol.73 (5), p.1209-1215</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-15f672f138753ad84bc50b49ee0946c64f93c3259b883d9bde9494482d5c9ab3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5282559$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1316940$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fletcher, Thomas M., III</creatorcontrib><creatorcontrib>Gray, Wayne L</creatorcontrib><title>Simian varicella virus: characterization of virion and infected cell polypeptides and the antigenic cross-reactivity with varicella-zoster virus</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Department of Microbiology and Immunology, 4301 West Markham Street, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, U.S.A.
Simian varicella virus (SVV) causes a varicella-like disease in non-human primates. In this study, SVV virions were purified from SVV-infected BSC-1 cells by zonal and differential gradient centrifugation and the virion polypeptide composition was analysed by SDS-PAGE. SVV virions had a buoyant density of 1.21 g/ml, identical to the value obtained for varicella-zoster virus (VZV) virions purified by the same method. Electron microscopy of the concentrated SVV virions revealed characteristic herpesvirus morphology. SVV virions consisted of at least 30 polypeptide species ranging from 16K to >200K. The electrophoretic profiles of radiolabelled SVV and VZV virion polypeptides were very similar. Immunoprecipitations of solubilized SVV-infected cell preparations using SVV immune sera revealed at least 18 viral polypeptides with an M r range of 12K to 142K and six glycoproteins ranging from 46K to 115K. In addition, extensive cross-reactivity between SVV and VZV proteins and glycoproteins was demonstrated by immunoprecipitation with heterologous immune sera. The high degree of antigenic relatedness between SVV and VZV provides further support for simian varicella as a model for VZV infections.
Received 14 October 1991;
accepted 24 January 1992.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cercopithecus aethiops</subject><subject>Cross Reactions</subject><subject>Disease Models, Animal</subject><subject>Erythrocebus patas</subject><subject>Evaluation Studies as Topic</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoproteins - chemistry</subject><subject>Glycoproteins - immunology</subject><subject>Hepatitis Delta Virus - chemistry</subject><subject>Hepatitis Delta Virus - immunology</subject><subject>Herpesvirus 3, Human - chemistry</subject><subject>Herpesvirus 3, Human - immunology</subject><subject>Microbiology</subject><subject>Precipitin Tests</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>varicella-zoster virus</subject><subject>Viral Proteins - chemistry</subject><subject>Viral Proteins - immunology</subject><subject>Virion - chemistry</subject><subject>Virion - immunology</subject><subject>Virion - isolation & purification</subject><subject>Virology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNqFUctuFDEQtBBR2AS-ACH5gFAuDn7OjLmhKAGkSBzI3fJ4PJlG88L2brT5Cj4Zz84qe-TULVd1VbsLofeMXjOq9WdKOSdMsJKUgijCONWv0IbJQpHc6tdo88J4gy5i_E0pk1KV5-g8vxVa0g36-wsGsCPe2QDO973FOwjb-AW7zgbrkg_wbBNMI57aBVo6OzYYxtZntMHLEJ6nfj_7OUHj4wFOnc81waMfwWEXphhJ8FkPdpD2-AlSd7Ikz1PMRqvzW3TW2j76d8d6iR7ubh9uvpP7n99-3Hy9J06KKhGm2qLkLRNVqYRtKlk7RWupvadaFq6QrRZOcKXrqhKNrhuvpZay4o1y2tbiEn1aZecw_dn6mMwA8bDN6KdtNCXXqhIl_y-RFbziBZeZKFbi4bfBt2YOMNiwN4yaJS-zpGGWNEwpjDJLXnnqw1F-Ww--Oc2sAWX84xG30dm-DXZ0EF9oKpsrtchcrbQOHrsnCN7kyw-QV6lhMvmwJ8d_ugiufA</recordid><startdate>19920501</startdate><enddate>19920501</enddate><creator>Fletcher, Thomas M., III</creator><creator>Gray, Wayne L</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19920501</creationdate><title>Simian varicella virus: characterization of virion and infected cell polypeptides and the antigenic cross-reactivity with varicella-zoster virus</title><author>Fletcher, Thomas M., III ; Gray, Wayne L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-15f672f138753ad84bc50b49ee0946c64f93c3259b883d9bde9494482d5c9ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cercopithecus aethiops</topic><topic>Cross Reactions</topic><topic>Disease Models, Animal</topic><topic>Erythrocebus patas</topic><topic>Evaluation Studies as Topic</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycoproteins - chemistry</topic><topic>Glycoproteins - immunology</topic><topic>Hepatitis Delta Virus - chemistry</topic><topic>Hepatitis Delta Virus - immunology</topic><topic>Herpesvirus 3, Human - chemistry</topic><topic>Herpesvirus 3, Human - immunology</topic><topic>Microbiology</topic><topic>Precipitin Tests</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>varicella-zoster virus</topic><topic>Viral Proteins - chemistry</topic><topic>Viral Proteins - immunology</topic><topic>Virion - chemistry</topic><topic>Virion - immunology</topic><topic>Virion - isolation & purification</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fletcher, Thomas M., III</creatorcontrib><creatorcontrib>Gray, Wayne L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fletcher, Thomas M., III</au><au>Gray, Wayne L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simian varicella virus: characterization of virion and infected cell polypeptides and the antigenic cross-reactivity with varicella-zoster virus</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1992-05-01</date><risdate>1992</risdate><volume>73</volume><issue>5</issue><spage>1209</spage><epage>1215</epage><pages>1209-1215</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>Department of Microbiology and Immunology, 4301 West Markham Street, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, U.S.A.
Simian varicella virus (SVV) causes a varicella-like disease in non-human primates. In this study, SVV virions were purified from SVV-infected BSC-1 cells by zonal and differential gradient centrifugation and the virion polypeptide composition was analysed by SDS-PAGE. SVV virions had a buoyant density of 1.21 g/ml, identical to the value obtained for varicella-zoster virus (VZV) virions purified by the same method. Electron microscopy of the concentrated SVV virions revealed characteristic herpesvirus morphology. SVV virions consisted of at least 30 polypeptide species ranging from 16K to >200K. The electrophoretic profiles of radiolabelled SVV and VZV virion polypeptides were very similar. Immunoprecipitations of solubilized SVV-infected cell preparations using SVV immune sera revealed at least 18 viral polypeptides with an M r range of 12K to 142K and six glycoproteins ranging from 46K to 115K. In addition, extensive cross-reactivity between SVV and VZV proteins and glycoproteins was demonstrated by immunoprecipitation with heterologous immune sera. The high degree of antigenic relatedness between SVV and VZV provides further support for simian varicella as a model for VZV infections.
Received 14 October 1991;
accepted 24 January 1992.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>1316940</pmid><doi>10.1099/0022-1317-73-5-1209</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 1992-05, Vol.73 (5), p.1209-1215 |
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| language | eng |
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| source | Freely Accessible Science Journals |
| subjects | Animals Biological and medical sciences Cercopithecus aethiops Cross Reactions Disease Models, Animal Erythrocebus patas Evaluation Studies as Topic Fundamental and applied biological sciences. Psychology Glycoproteins - chemistry Glycoproteins - immunology Hepatitis Delta Virus - chemistry Hepatitis Delta Virus - immunology Herpesvirus 3, Human - chemistry Herpesvirus 3, Human - immunology Microbiology Precipitin Tests Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains varicella-zoster virus Viral Proteins - chemistry Viral Proteins - immunology Virion - chemistry Virion - immunology Virion - isolation & purification Virology |
| title | Simian varicella virus: characterization of virion and infected cell polypeptides and the antigenic cross-reactivity with varicella-zoster virus |
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