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Liquid chromatography–tandem mass spectrometry analysis of oleuropein and its metabolite hydroxytyrosol in rat plasma and urine after oral administration
We describe a liquid chromatography–electrospray ionisation tandem mass spectrometry method for the qualitative and quantitative determination of the secoiridoid oleuropein and its bioactive metabolite hydroxytyrosol in rat plasma and urine. Samples were prepared by liquid–liquid extraction using et...
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Published in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2003-02, Vol.785 (1), p.47-56 |
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container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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creator | Del Boccio, Piero Di Deo, Antonietta De Curtis, Amalia Celli, Nicola Iacoviello, Licia Rotilio, Domenico |
description | We describe a liquid chromatography–electrospray ionisation tandem mass spectrometry method for the qualitative and quantitative determination of the secoiridoid oleuropein and its bioactive metabolite hydroxytyrosol in rat plasma and urine. Samples were prepared by liquid–liquid extraction using ethyl acetate with a recovery for both compounds of about 100% in plasma and about 60% in urine. The chromatographic separation was performed with a RP-ODS column using a water–acetonitrile linear gradient. The calibration curve was linear for both biophenols over the range 2.5–1000 ng/ml (LOD 1.25 ng/ml) for plasma and 5–1000 ng/ml (LOD 2.5 ng/ml) for urine. Plasma concentrations of oleuropein and hydroxytyrosol were measured after oral administration of a single dose (100 mg/kg) of oleuropein. Analysis of treated rat plasma showed the presence of unmodified oleuropein, reaching a peak value of 200 ng/ml within 2 h, with a small amount of hydroxytyrosol, whereas in urine, both compounds were mainly found as glucuronides. |
doi_str_mv | 10.1016/S1570-0232(02)00853-X |
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Samples were prepared by liquid–liquid extraction using ethyl acetate with a recovery for both compounds of about 100% in plasma and about 60% in urine. The chromatographic separation was performed with a RP-ODS column using a water–acetonitrile linear gradient. The calibration curve was linear for both biophenols over the range 2.5–1000 ng/ml (LOD 1.25 ng/ml) for plasma and 5–1000 ng/ml (LOD 2.5 ng/ml) for urine. Plasma concentrations of oleuropein and hydroxytyrosol were measured after oral administration of a single dose (100 mg/kg) of oleuropein. Analysis of treated rat plasma showed the presence of unmodified oleuropein, reaching a peak value of 200 ng/ml within 2 h, with a small amount of hydroxytyrosol, whereas in urine, both compounds were mainly found as glucuronides.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/S1570-0232(02)00853-X</identifier><identifier>PMID: 12535837</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Administration, Oral ; Animals ; Calibration ; Hydroxytyrosol ; Iridoids ; Oleuropein ; Phenylethyl Alcohol - analogs & derivatives ; Phenylethyl Alcohol - analysis ; Phenylethyl Alcohol - blood ; Phenylethyl Alcohol - urine ; Pyrans - administration & dosage ; Pyrans - analysis ; Pyrans - blood ; Pyrans - urine ; Rats ; Sensitivity and Specificity</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2003-02, Vol.785 (1), p.47-56</ispartof><rights>2002 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-c9765f60aee4cfcc7e372fcd7220a0f255677b2f3c41c1536a4930474df2e0663</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12535837$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Del Boccio, Piero</creatorcontrib><creatorcontrib>Di Deo, Antonietta</creatorcontrib><creatorcontrib>De Curtis, Amalia</creatorcontrib><creatorcontrib>Celli, Nicola</creatorcontrib><creatorcontrib>Iacoviello, Licia</creatorcontrib><creatorcontrib>Rotilio, Domenico</creatorcontrib><title>Liquid chromatography–tandem mass spectrometry analysis of oleuropein and its metabolite hydroxytyrosol in rat plasma and urine after oral administration</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>We describe a liquid chromatography–electrospray ionisation tandem mass spectrometry method for the qualitative and quantitative determination of the secoiridoid oleuropein and its bioactive metabolite hydroxytyrosol in rat plasma and urine. Samples were prepared by liquid–liquid extraction using ethyl acetate with a recovery for both compounds of about 100% in plasma and about 60% in urine. The chromatographic separation was performed with a RP-ODS column using a water–acetonitrile linear gradient. The calibration curve was linear for both biophenols over the range 2.5–1000 ng/ml (LOD 1.25 ng/ml) for plasma and 5–1000 ng/ml (LOD 2.5 ng/ml) for urine. Plasma concentrations of oleuropein and hydroxytyrosol were measured after oral administration of a single dose (100 mg/kg) of oleuropein. Analysis of treated rat plasma showed the presence of unmodified oleuropein, reaching a peak value of 200 ng/ml within 2 h, with a small amount of hydroxytyrosol, whereas in urine, both compounds were mainly found as glucuronides.</description><subject>Administration, Oral</subject><subject>Animals</subject><subject>Calibration</subject><subject>Hydroxytyrosol</subject><subject>Iridoids</subject><subject>Oleuropein</subject><subject>Phenylethyl Alcohol - analogs & derivatives</subject><subject>Phenylethyl Alcohol - analysis</subject><subject>Phenylethyl Alcohol - blood</subject><subject>Phenylethyl Alcohol - urine</subject><subject>Pyrans - administration & dosage</subject><subject>Pyrans - analysis</subject><subject>Pyrans - blood</subject><subject>Pyrans - urine</subject><subject>Rats</subject><subject>Sensitivity and Specificity</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqFkc1uFDEMxyMEoqXwCKCcED0M5GNmsntCVUUBaSUOgLS3yJs4bFBmMk0yiLnxDhx5O56EdHcRRy62Zf9sy_4T8pSzl5zx_tVH3inWMCHFCyYuGVt1stneI-d8pWQjVb-9X-O_yBl5lPNXxrhiSj4kZ1x0sltJdU5-bfzt7C01-xQHKPFLgmm__P7xs8BocaAD5EzzhKbUOpa0UBghLNlnGh2NAecUJ_RjTVvqS6YVgl0MviDdLzbF70tZUswx0AolKHQKkAc48HPyI1JwBRONCQIFO_jR51I5H8fH5IGDkPHJyV-QzzdvPl2_azYf3r6_vto0Rq5Zacxa9Z3rGSC2xhmjUCrhjFVCMGBOdF2v1E44aVpueCd7aNeStaq1TiDre3lBnh_nTinezpiLHnw2GAKMGOeslVj3QrZtBbsjaOpFOaHTU_IDpEVzpu9U0QdV9N3Lq9EHVfS29j07LZh3A9p_XScZKvD6CGA985vHpLPxOBq0PtXXaxv9f1b8Adw4oq8</recordid><startdate>20030225</startdate><enddate>20030225</enddate><creator>Del Boccio, Piero</creator><creator>Di Deo, Antonietta</creator><creator>De Curtis, Amalia</creator><creator>Celli, Nicola</creator><creator>Iacoviello, Licia</creator><creator>Rotilio, Domenico</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030225</creationdate><title>Liquid chromatography–tandem mass spectrometry analysis of oleuropein and its metabolite hydroxytyrosol in rat plasma and urine after oral administration</title><author>Del Boccio, Piero ; Di Deo, Antonietta ; De Curtis, Amalia ; Celli, Nicola ; Iacoviello, Licia ; Rotilio, Domenico</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-c9765f60aee4cfcc7e372fcd7220a0f255677b2f3c41c1536a4930474df2e0663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Administration, Oral</topic><topic>Animals</topic><topic>Calibration</topic><topic>Hydroxytyrosol</topic><topic>Iridoids</topic><topic>Oleuropein</topic><topic>Phenylethyl Alcohol - analogs & derivatives</topic><topic>Phenylethyl Alcohol - analysis</topic><topic>Phenylethyl Alcohol - blood</topic><topic>Phenylethyl Alcohol - urine</topic><topic>Pyrans - administration & dosage</topic><topic>Pyrans - analysis</topic><topic>Pyrans - blood</topic><topic>Pyrans - urine</topic><topic>Rats</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Del Boccio, Piero</creatorcontrib><creatorcontrib>Di Deo, Antonietta</creatorcontrib><creatorcontrib>De Curtis, Amalia</creatorcontrib><creatorcontrib>Celli, Nicola</creatorcontrib><creatorcontrib>Iacoviello, Licia</creatorcontrib><creatorcontrib>Rotilio, Domenico</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. 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B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2003-02-25</date><risdate>2003</risdate><volume>785</volume><issue>1</issue><spage>47</spage><epage>56</epage><pages>47-56</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>We describe a liquid chromatography–electrospray ionisation tandem mass spectrometry method for the qualitative and quantitative determination of the secoiridoid oleuropein and its bioactive metabolite hydroxytyrosol in rat plasma and urine. Samples were prepared by liquid–liquid extraction using ethyl acetate with a recovery for both compounds of about 100% in plasma and about 60% in urine. The chromatographic separation was performed with a RP-ODS column using a water–acetonitrile linear gradient. The calibration curve was linear for both biophenols over the range 2.5–1000 ng/ml (LOD 1.25 ng/ml) for plasma and 5–1000 ng/ml (LOD 2.5 ng/ml) for urine. Plasma concentrations of oleuropein and hydroxytyrosol were measured after oral administration of a single dose (100 mg/kg) of oleuropein. Analysis of treated rat plasma showed the presence of unmodified oleuropein, reaching a peak value of 200 ng/ml within 2 h, with a small amount of hydroxytyrosol, whereas in urine, both compounds were mainly found as glucuronides.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>12535837</pmid><doi>10.1016/S1570-0232(02)00853-X</doi><tpages>10</tpages></addata></record> |
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subjects | Administration, Oral Animals Calibration Hydroxytyrosol Iridoids Oleuropein Phenylethyl Alcohol - analogs & derivatives Phenylethyl Alcohol - analysis Phenylethyl Alcohol - blood Phenylethyl Alcohol - urine Pyrans - administration & dosage Pyrans - analysis Pyrans - blood Pyrans - urine Rats Sensitivity and Specificity |
title | Liquid chromatography–tandem mass spectrometry analysis of oleuropein and its metabolite hydroxytyrosol in rat plasma and urine after oral administration |
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