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Isolation and biochemical characterisation of enterocins produced by enterococci from different sources
Aims: Comparison of enterocins produced by six Enterococcus faecium strains and one Ent. faecalis strain isolated from different origin with regard to their microbiological and biochemical characteristics in view of their technological potential and practical use. Methods and Results: The seven ente...
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Published in: | Journal of applied microbiology 2003-01, Vol.94 (2), p.214-229 |
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description | Aims: Comparison of enterocins produced by six Enterococcus faecium strains and one Ent. faecalis strain isolated from different origin with regard to their microbiological and biochemical characteristics in view of their technological potential and practical use.
Methods and Results: The seven enterococci were sensitive to the glycopeptide antibiotics vancomycin and teicoplanin and did not show haemolytic activity. The absence of the glycopeptide‐resistant genotypes and the genes involved in the production of the lantibiotic cytolysin was confirmed by PCR. The enterocins were active towards Listeria innocua and other lactic acid bacteria. Their temperature stability was dependent on the pH and their activity was higher at acidic pH. A bactericidal and bacteriolytic effect was shown. PCR analyses revealed that the gene of enterocin A was present in the genome of Ent. faecium CCM 4231, Ent. faecium 306 I.2.20 and Ent. faecalis Y; both enterocin A and B genes were present in the genome of Ent. faecium LMG 11423T, Ent. faecium RZS C5 and Ent. faecium RZS C13. Enterocin P was detected in the genome of Ent. faecium RZS C5 and Ent. faecium RZS C13. No signal was found for Ent. faecium SF 68. Enterocins from Ent. faecium RZS C5, Ent. faecium RZS C13 and Ent. faecium SF 68 were purified to homogeneity.
Conclusions: Ent. faecium RZS C5 and Ent. faecium RZS C13 produced an enterocin with a molecular mass of 5460 and 5477 Da, respectively, which was in the range of that of enterocin B. The amino acid sequence analysis of the enterocin from Ent. faecium RZS C13 revealed 24 N‐terminal residues, which were identical to those of enterocin B. The enterocin from Ent. faecium SF 68 had a molecular mass of 4488 Da, which did not correspond to any enterocin known so far.
Significance and Impact of the Study: The number of characterized enterocins is increasing. As this type of work is tedious and time‐consuming, it may be interesting to include PCR as a first step to know if the Enterococcus strain in study produces either a known or a new enterocin. Also, it is important to check the absence of cytolysin and resistance to vancomycin for a further application of the Enterococcus strain in food or health applications. |
doi_str_mv | 10.1046/j.1365-2672.2003.01823.x |
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Methods and Results: The seven enterococci were sensitive to the glycopeptide antibiotics vancomycin and teicoplanin and did not show haemolytic activity. The absence of the glycopeptide‐resistant genotypes and the genes involved in the production of the lantibiotic cytolysin was confirmed by PCR. The enterocins were active towards Listeria innocua and other lactic acid bacteria. Their temperature stability was dependent on the pH and their activity was higher at acidic pH. A bactericidal and bacteriolytic effect was shown. PCR analyses revealed that the gene of enterocin A was present in the genome of Ent. faecium CCM 4231, Ent. faecium 306 I.2.20 and Ent. faecalis Y; both enterocin A and B genes were present in the genome of Ent. faecium LMG 11423T, Ent. faecium RZS C5 and Ent. faecium RZS C13. Enterocin P was detected in the genome of Ent. faecium RZS C5 and Ent. faecium RZS C13. No signal was found for Ent. faecium SF 68. Enterocins from Ent. faecium RZS C5, Ent. faecium RZS C13 and Ent. faecium SF 68 were purified to homogeneity.
Conclusions: Ent. faecium RZS C5 and Ent. faecium RZS C13 produced an enterocin with a molecular mass of 5460 and 5477 Da, respectively, which was in the range of that of enterocin B. The amino acid sequence analysis of the enterocin from Ent. faecium RZS C13 revealed 24 N‐terminal residues, which were identical to those of enterocin B. The enterocin from Ent. faecium SF 68 had a molecular mass of 4488 Da, which did not correspond to any enterocin known so far.
Significance and Impact of the Study: The number of characterized enterocins is increasing. As this type of work is tedious and time‐consuming, it may be interesting to include PCR as a first step to know if the Enterococcus strain in study produces either a known or a new enterocin. Also, it is important to check the absence of cytolysin and resistance to vancomycin for a further application of the Enterococcus strain in food or health applications.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1046/j.1365-2672.2003.01823.x</identifier><identifier>PMID: 12534813</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Animals ; Bacteriocin ; Bacteriocins - genetics ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; Bridged-Ring Compounds - chemistry ; Bridged-Ring Compounds - isolation & purification ; Electrophoresis, Polyacrylamide Gel - methods ; enterocin ; Enterococcus ; Enterococcus - genetics ; Enterococcus - metabolism ; Enterococcus faecalis - genetics ; Enterococcus faecalis - metabolism ; Enterococcus faecium - genetics ; Enterococcus faecium - metabolism ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial - genetics ; Hemolysis ; Horses - microbiology ; Hot Temperature ; Humans ; Hydrogen-Ion Concentration ; Microbial Sensitivity Tests - methods ; Miscellaneous ; Mission oriented research ; polymerase chain reaction ; Polymerase Chain Reaction - methods ; purification ; Sheep - microbiology ; Vancomycin Resistance</subject><ispartof>Journal of applied microbiology, 2003-01, Vol.94 (2), p.214-229</ispartof><rights>2003 INIST-CNRS</rights><rights>Copyright Blackwell Science Ltd. 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4533-c54f46581fb6db1304d581f47cbacac24de4d86b7dededd180502d309eb9f59c3</citedby><cites>FETCH-LOGICAL-c4533-c54f46581fb6db1304d581f47cbacac24de4d86b7dededd180502d309eb9f59c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14640435$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12534813$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Foulquié Moreno, M.R.</creatorcontrib><creatorcontrib>Callewaert, R.</creatorcontrib><creatorcontrib>Devreese, B.</creatorcontrib><creatorcontrib>Van Beeumen, J.</creatorcontrib><creatorcontrib>De Vuyst, L.</creatorcontrib><title>Isolation and biochemical characterisation of enterocins produced by enterococci from different sources</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims: Comparison of enterocins produced by six Enterococcus faecium strains and one Ent. faecalis strain isolated from different origin with regard to their microbiological and biochemical characteristics in view of their technological potential and practical use.
Methods and Results: The seven enterococci were sensitive to the glycopeptide antibiotics vancomycin and teicoplanin and did not show haemolytic activity. The absence of the glycopeptide‐resistant genotypes and the genes involved in the production of the lantibiotic cytolysin was confirmed by PCR. The enterocins were active towards Listeria innocua and other lactic acid bacteria. Their temperature stability was dependent on the pH and their activity was higher at acidic pH. A bactericidal and bacteriolytic effect was shown. PCR analyses revealed that the gene of enterocin A was present in the genome of Ent. faecium CCM 4231, Ent. faecium 306 I.2.20 and Ent. faecalis Y; both enterocin A and B genes were present in the genome of Ent. faecium LMG 11423T, Ent. faecium RZS C5 and Ent. faecium RZS C13. Enterocin P was detected in the genome of Ent. faecium RZS C5 and Ent. faecium RZS C13. No signal was found for Ent. faecium SF 68. Enterocins from Ent. faecium RZS C5, Ent. faecium RZS C13 and Ent. faecium SF 68 were purified to homogeneity.
Conclusions: Ent. faecium RZS C5 and Ent. faecium RZS C13 produced an enterocin with a molecular mass of 5460 and 5477 Da, respectively, which was in the range of that of enterocin B. The amino acid sequence analysis of the enterocin from Ent. faecium RZS C13 revealed 24 N‐terminal residues, which were identical to those of enterocin B. The enterocin from Ent. faecium SF 68 had a molecular mass of 4488 Da, which did not correspond to any enterocin known so far.
Significance and Impact of the Study: The number of characterized enterocins is increasing. As this type of work is tedious and time‐consuming, it may be interesting to include PCR as a first step to know if the Enterococcus strain in study produces either a known or a new enterocin. Also, it is important to check the absence of cytolysin and resistance to vancomycin for a further application of the Enterococcus strain in food or health applications.</description><subject>Animals</subject><subject>Bacteriocin</subject><subject>Bacteriocins - genetics</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>Bridged-Ring Compounds - chemistry</subject><subject>Bridged-Ring Compounds - isolation & purification</subject><subject>Electrophoresis, Polyacrylamide Gel - methods</subject><subject>enterocin</subject><subject>Enterococcus</subject><subject>Enterococcus - genetics</subject><subject>Enterococcus - metabolism</subject><subject>Enterococcus faecalis - genetics</subject><subject>Enterococcus faecalis - metabolism</subject><subject>Enterococcus faecium - genetics</subject><subject>Enterococcus faecium - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial - genetics</subject><subject>Hemolysis</subject><subject>Horses - microbiology</subject><subject>Hot Temperature</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Microbial Sensitivity Tests - methods</subject><subject>Miscellaneous</subject><subject>Mission oriented research</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>purification</subject><subject>Sheep - microbiology</subject><subject>Vancomycin Resistance</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqNkU1P3DAQhq2qVYFt_wKyKpVbUn8nOXBAiFIqql7gbDljG7xKYrA3gv33ddhtkXpp5YNnxs87mvGLEKakpkSoL-uaciUrphpWM0J4TWjLeP38Bh3-eXj7EotKkoYdoKOc14RQTqR6jw4ok1y0lB-iu6scB7MJccJmsrgPEe7dGMAMGO5NMrBxKeQdED12U8kjhCnjhxTtDK5otr_LESBgn-KIbfDepVLGOc4JXP6A3nkzZPdxf6_Q7deLm_Nv1fXPy6vzs-sKhOS8Aim8ULKlvle2L-MKuySigd6AASasE7ZVfWNdOZa2RBJmOelc33nZAV-hk13fMt7j7PJGjyGDGwYzuThn3bBOiYa0_wRpqxreNayAn_4C12WlqSyhGWedZKJ86wq1OwhSzDk5rx9SGE3aakr0Yple68UZvTijF8v0i2X6uUiP9_3nfnT2Vbj3qACf94DJxRefzAQhv3JCCSK4LNzpjnsKg9v-9wD6-9mPJeK_ABZls0U</recordid><startdate>20030101</startdate><enddate>20030101</enddate><creator>Foulquié Moreno, M.R.</creator><creator>Callewaert, R.</creator><creator>Devreese, B.</creator><creator>Van Beeumen, J.</creator><creator>De Vuyst, L.</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20030101</creationdate><title>Isolation and biochemical characterisation of enterocins produced by enterococci from different sources</title><author>Foulquié Moreno, M.R. ; Callewaert, R. ; Devreese, B. ; Van Beeumen, J. ; De Vuyst, L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4533-c54f46581fb6db1304d581f47cbacac24de4d86b7dededd180502d309eb9f59c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Bacteriocin</topic><topic>Bacteriocins - genetics</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>Bridged-Ring Compounds - chemistry</topic><topic>Bridged-Ring Compounds - isolation & purification</topic><topic>Electrophoresis, Polyacrylamide Gel - methods</topic><topic>enterocin</topic><topic>Enterococcus</topic><topic>Enterococcus - genetics</topic><topic>Enterococcus - metabolism</topic><topic>Enterococcus faecalis - genetics</topic><topic>Enterococcus faecalis - metabolism</topic><topic>Enterococcus faecium - genetics</topic><topic>Enterococcus faecium - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial - genetics</topic><topic>Hemolysis</topic><topic>Horses - microbiology</topic><topic>Hot Temperature</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Microbial Sensitivity Tests - methods</topic><topic>Miscellaneous</topic><topic>Mission oriented research</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>purification</topic><topic>Sheep - microbiology</topic><topic>Vancomycin Resistance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Foulquié Moreno, M.R.</creatorcontrib><creatorcontrib>Callewaert, R.</creatorcontrib><creatorcontrib>Devreese, B.</creatorcontrib><creatorcontrib>Van Beeumen, J.</creatorcontrib><creatorcontrib>De Vuyst, L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Foulquié Moreno, M.R.</au><au>Callewaert, R.</au><au>Devreese, B.</au><au>Van Beeumen, J.</au><au>De Vuyst, L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and biochemical characterisation of enterocins produced by enterococci from different sources</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2003-01-01</date><risdate>2003</risdate><volume>94</volume><issue>2</issue><spage>214</spage><epage>229</epage><pages>214-229</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>Aims: Comparison of enterocins produced by six Enterococcus faecium strains and one Ent. faecalis strain isolated from different origin with regard to their microbiological and biochemical characteristics in view of their technological potential and practical use.
Methods and Results: The seven enterococci were sensitive to the glycopeptide antibiotics vancomycin and teicoplanin and did not show haemolytic activity. The absence of the glycopeptide‐resistant genotypes and the genes involved in the production of the lantibiotic cytolysin was confirmed by PCR. The enterocins were active towards Listeria innocua and other lactic acid bacteria. Their temperature stability was dependent on the pH and their activity was higher at acidic pH. A bactericidal and bacteriolytic effect was shown. PCR analyses revealed that the gene of enterocin A was present in the genome of Ent. faecium CCM 4231, Ent. faecium 306 I.2.20 and Ent. faecalis Y; both enterocin A and B genes were present in the genome of Ent. faecium LMG 11423T, Ent. faecium RZS C5 and Ent. faecium RZS C13. Enterocin P was detected in the genome of Ent. faecium RZS C5 and Ent. faecium RZS C13. No signal was found for Ent. faecium SF 68. Enterocins from Ent. faecium RZS C5, Ent. faecium RZS C13 and Ent. faecium SF 68 were purified to homogeneity.
Conclusions: Ent. faecium RZS C5 and Ent. faecium RZS C13 produced an enterocin with a molecular mass of 5460 and 5477 Da, respectively, which was in the range of that of enterocin B. The amino acid sequence analysis of the enterocin from Ent. faecium RZS C13 revealed 24 N‐terminal residues, which were identical to those of enterocin B. The enterocin from Ent. faecium SF 68 had a molecular mass of 4488 Da, which did not correspond to any enterocin known so far.
Significance and Impact of the Study: The number of characterized enterocins is increasing. As this type of work is tedious and time‐consuming, it may be interesting to include PCR as a first step to know if the Enterococcus strain in study produces either a known or a new enterocin. Also, it is important to check the absence of cytolysin and resistance to vancomycin for a further application of the Enterococcus strain in food or health applications.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>12534813</pmid><doi>10.1046/j.1365-2672.2003.01823.x</doi><tpages>16</tpages></addata></record> |
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source | Alma/SFX Local Collection |
subjects | Animals Bacteriocin Bacteriocins - genetics Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Bridged-Ring Compounds - chemistry Bridged-Ring Compounds - isolation & purification Electrophoresis, Polyacrylamide Gel - methods enterocin Enterococcus Enterococcus - genetics Enterococcus - metabolism Enterococcus faecalis - genetics Enterococcus faecalis - metabolism Enterococcus faecium - genetics Enterococcus faecium - metabolism Fundamental and applied biological sciences. Psychology Genes, Bacterial - genetics Hemolysis Horses - microbiology Hot Temperature Humans Hydrogen-Ion Concentration Microbial Sensitivity Tests - methods Miscellaneous Mission oriented research polymerase chain reaction Polymerase Chain Reaction - methods purification Sheep - microbiology Vancomycin Resistance |
title | Isolation and biochemical characterisation of enterocins produced by enterococci from different sources |
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