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Testis-Specific Expression of Rat Mitochondrial Glycerol-3-Phosphate Dehydrogenase in Haploid Male Germ Cells
Mitochondrial glycerol-3-phosphate dehydrogenase (mGPDH) is regulated by multiple promoters in a tissue-specific manner. We characterized the testis-specific promoter C of the mGPDH gene and investigated the cellular localization of mGPDH within the testis. Electrophoretic mobility shift experiments...
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| Published in: | Biology of reproduction 2003-02, Vol.68 (2), p.699-707 |
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| Main Authors: | , , , , , , |
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| Language: | English |
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| container_end_page | 707 |
| container_issue | 2 |
| container_start_page | 699 |
| container_title | Biology of reproduction |
| container_volume | 68 |
| creator | WEITZEL, Joachim M SHIRYAEVA, Natalia B MIDDENDORFF, Ralf BALVERS, Marga RADTKE, Christiane LVELL, Richard SEITZ, Hans J |
| description | Mitochondrial glycerol-3-phosphate dehydrogenase (mGPDH) is regulated by multiple promoters in a tissue-specific manner. We
characterized the testis-specific promoter C of the mGPDH gene and investigated the cellular localization of mGPDH within
the testis. Electrophoretic mobility shift experiments identified a cAMP-response element (CRE) site at â57 that was active
in the testis. An in vitro-translated CRE modulator (CREM) protein was able to bind this CRE site, and an anti-CREM antibody
interfered with this complex. Ectopic expression of the testis-specific transcriptional activator CREMÏ and protein kinase
A in human hepatocarcinoma HepG2 cells activated a promoter C-driven luciferase construct in transient transfection experiments.
Furthermore, mGPDH expression was undetectable in testis of CREM-deficient mice. The cellular localization of mGPDH expression
and translation in adult rat testis was determined by in situ hybridization and immunohistochemistry techniques. The mGPDH
transcripts were detected solely in postmeiotic germ cells. Expression of mGPDH was restricted from round spermatids to early
elongating spermatids. The mGPDH protein was delayed in postmeiotic germ cells, restricted from late elongating spermatids
to mature spermatids. Our results indicate that rat mGPDH is expressed by a testis-specific promoter from haploid male germ
cells in a stage-specific manner. |
| doi_str_mv | 10.1095/biolreprod.102.008540 |
| format | article |
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characterized the testis-specific promoter C of the mGPDH gene and investigated the cellular localization of mGPDH within
the testis. Electrophoretic mobility shift experiments identified a cAMP-response element (CRE) site at â57 that was active
in the testis. An in vitro-translated CRE modulator (CREM) protein was able to bind this CRE site, and an anti-CREM antibody
interfered with this complex. Ectopic expression of the testis-specific transcriptional activator CREMÏ and protein kinase
A in human hepatocarcinoma HepG2 cells activated a promoter C-driven luciferase construct in transient transfection experiments.
Furthermore, mGPDH expression was undetectable in testis of CREM-deficient mice. The cellular localization of mGPDH expression
and translation in adult rat testis was determined by in situ hybridization and immunohistochemistry techniques. The mGPDH
transcripts were detected solely in postmeiotic germ cells. Expression of mGPDH was restricted from round spermatids to early
elongating spermatids. The mGPDH protein was delayed in postmeiotic germ cells, restricted from late elongating spermatids
to mature spermatids. Our results indicate that rat mGPDH is expressed by a testis-specific promoter from haploid male germ
cells in a stage-specific manner.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod.102.008540</identifier><identifier>PMID: 12533437</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Base Sequence - genetics ; Biological and medical sciences ; Electrophoresis ; Fundamental and applied biological sciences. Psychology ; Glycerolphosphate Dehydrogenase - genetics ; Glycerolphosphate Dehydrogenase - metabolism ; Humans ; Immunohistochemistry ; In Situ Hybridization ; Male ; Mammalian male genital system ; Mice ; Mitochondria - enzymology ; Molecular Sequence Data ; Morphology. Physiology ; Promoter Regions, Genetic - genetics ; Rats ; Rats, Wistar ; Spermatozoa - metabolism ; Testis - enzymology ; Transfection ; Tumor Cells, Cultured ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2003-02, Vol.68 (2), p.699-707</ispartof><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14491678$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12533437$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WEITZEL, Joachim M</creatorcontrib><creatorcontrib>SHIRYAEVA, Natalia B</creatorcontrib><creatorcontrib>MIDDENDORFF, Ralf</creatorcontrib><creatorcontrib>BALVERS, Marga</creatorcontrib><creatorcontrib>RADTKE, Christiane</creatorcontrib><creatorcontrib>LVELL, Richard</creatorcontrib><creatorcontrib>SEITZ, Hans J</creatorcontrib><title>Testis-Specific Expression of Rat Mitochondrial Glycerol-3-Phosphate Dehydrogenase in Haploid Male Germ Cells</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Mitochondrial glycerol-3-phosphate dehydrogenase (mGPDH) is regulated by multiple promoters in a tissue-specific manner. We
characterized the testis-specific promoter C of the mGPDH gene and investigated the cellular localization of mGPDH within
the testis. Electrophoretic mobility shift experiments identified a cAMP-response element (CRE) site at â57 that was active
in the testis. An in vitro-translated CRE modulator (CREM) protein was able to bind this CRE site, and an anti-CREM antibody
interfered with this complex. Ectopic expression of the testis-specific transcriptional activator CREMÏ and protein kinase
A in human hepatocarcinoma HepG2 cells activated a promoter C-driven luciferase construct in transient transfection experiments.
Furthermore, mGPDH expression was undetectable in testis of CREM-deficient mice. The cellular localization of mGPDH expression
and translation in adult rat testis was determined by in situ hybridization and immunohistochemistry techniques. The mGPDH
transcripts were detected solely in postmeiotic germ cells. Expression of mGPDH was restricted from round spermatids to early
elongating spermatids. The mGPDH protein was delayed in postmeiotic germ cells, restricted from late elongating spermatids
to mature spermatids. Our results indicate that rat mGPDH is expressed by a testis-specific promoter from haploid male germ
cells in a stage-specific manner.</description><subject>Animals</subject><subject>Base Sequence - genetics</subject><subject>Biological and medical sciences</subject><subject>Electrophoresis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycerolphosphate Dehydrogenase - genetics</subject><subject>Glycerolphosphate Dehydrogenase - metabolism</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Mice</subject><subject>Mitochondria - enzymology</subject><subject>Molecular Sequence Data</subject><subject>Morphology. Physiology</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Spermatozoa - metabolism</subject><subject>Testis - enzymology</subject><subject>Transfection</subject><subject>Tumor Cells, Cultured</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNpF0N9r2zAQB3BRNpo065_QoZf1zZ1-WbYeR9qmg5aNNns2inSqNWTLkxy8_Pc1NCVPx3EfjrsvQleU3FCiyu87H0OCIUU79-yGkLoU5AwtaclUUTFZf0JLQogsOJd8gS5y_ksIFZzxc7SgrORc8GqJui3k0efiZQDjnTf47v-QIGcfexwdftYjfvJjNG3sbfI64E04GEgxFLz43cY8tHoEfAvtwab4Cr3OgH2PH_QQorf4SQfAG0gdXkMI-Qv67HTIcHmsK_Tn_m67figef21-rn88Fi2T1ViA5coSUVtGqLNSUEd2tTaGMSGd0owTJXeirJxj80C4EpyQvDKWUV1Tq_gKXb_vnfP5t58_bDqfzXyB7iHuc1MxJaUgdIZfj3C_68A2Q_KdTofmI6AZfDsCnY0OLune-HxyQigqq_rkWv_aTj5BkzsdwryWN9M0ybphjVSKvwGUGYUn</recordid><startdate>20030201</startdate><enddate>20030201</enddate><creator>WEITZEL, Joachim M</creator><creator>SHIRYAEVA, Natalia B</creator><creator>MIDDENDORFF, Ralf</creator><creator>BALVERS, Marga</creator><creator>RADTKE, Christiane</creator><creator>LVELL, Richard</creator><creator>SEITZ, Hans J</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20030201</creationdate><title>Testis-Specific Expression of Rat Mitochondrial Glycerol-3-Phosphate Dehydrogenase in Haploid Male Germ Cells</title><author>WEITZEL, Joachim M ; SHIRYAEVA, Natalia B ; MIDDENDORFF, Ralf ; BALVERS, Marga ; RADTKE, Christiane ; LVELL, Richard ; SEITZ, Hans J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h267t-ed39d048d201fd641f0b8acc2246f9a23096b457ff20b84f5ef4637cd21a81d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Base Sequence - genetics</topic><topic>Biological and medical sciences</topic><topic>Electrophoresis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycerolphosphate Dehydrogenase - genetics</topic><topic>Glycerolphosphate Dehydrogenase - metabolism</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Mice</topic><topic>Mitochondria - enzymology</topic><topic>Molecular Sequence Data</topic><topic>Morphology. Physiology</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Spermatozoa - metabolism</topic><topic>Testis - enzymology</topic><topic>Transfection</topic><topic>Tumor Cells, Cultured</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WEITZEL, Joachim M</creatorcontrib><creatorcontrib>SHIRYAEVA, Natalia B</creatorcontrib><creatorcontrib>MIDDENDORFF, Ralf</creatorcontrib><creatorcontrib>BALVERS, Marga</creatorcontrib><creatorcontrib>RADTKE, Christiane</creatorcontrib><creatorcontrib>LVELL, Richard</creatorcontrib><creatorcontrib>SEITZ, Hans J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WEITZEL, Joachim M</au><au>SHIRYAEVA, Natalia B</au><au>MIDDENDORFF, Ralf</au><au>BALVERS, Marga</au><au>RADTKE, Christiane</au><au>LVELL, Richard</au><au>SEITZ, Hans J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Testis-Specific Expression of Rat Mitochondrial Glycerol-3-Phosphate Dehydrogenase in Haploid Male Germ Cells</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2003-02-01</date><risdate>2003</risdate><volume>68</volume><issue>2</issue><spage>699</spage><epage>707</epage><pages>699-707</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Mitochondrial glycerol-3-phosphate dehydrogenase (mGPDH) is regulated by multiple promoters in a tissue-specific manner. We
characterized the testis-specific promoter C of the mGPDH gene and investigated the cellular localization of mGPDH within
the testis. Electrophoretic mobility shift experiments identified a cAMP-response element (CRE) site at â57 that was active
in the testis. An in vitro-translated CRE modulator (CREM) protein was able to bind this CRE site, and an anti-CREM antibody
interfered with this complex. Ectopic expression of the testis-specific transcriptional activator CREMÏ and protein kinase
A in human hepatocarcinoma HepG2 cells activated a promoter C-driven luciferase construct in transient transfection experiments.
Furthermore, mGPDH expression was undetectable in testis of CREM-deficient mice. The cellular localization of mGPDH expression
and translation in adult rat testis was determined by in situ hybridization and immunohistochemistry techniques. The mGPDH
transcripts were detected solely in postmeiotic germ cells. Expression of mGPDH was restricted from round spermatids to early
elongating spermatids. The mGPDH protein was delayed in postmeiotic germ cells, restricted from late elongating spermatids
to mature spermatids. Our results indicate that rat mGPDH is expressed by a testis-specific promoter from haploid male germ
cells in a stage-specific manner.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>12533437</pmid><doi>10.1095/biolreprod.102.008540</doi><tpages>9</tpages></addata></record> |
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| ispartof | Biology of reproduction, 2003-02, Vol.68 (2), p.699-707 |
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| source | Oxford Journals Online |
| subjects | Animals Base Sequence - genetics Biological and medical sciences Electrophoresis Fundamental and applied biological sciences. Psychology Glycerolphosphate Dehydrogenase - genetics Glycerolphosphate Dehydrogenase - metabolism Humans Immunohistochemistry In Situ Hybridization Male Mammalian male genital system Mice Mitochondria - enzymology Molecular Sequence Data Morphology. Physiology Promoter Regions, Genetic - genetics Rats Rats, Wistar Spermatozoa - metabolism Testis - enzymology Transfection Tumor Cells, Cultured Vertebrates: reproduction |
| title | Testis-Specific Expression of Rat Mitochondrial Glycerol-3-Phosphate Dehydrogenase in Haploid Male Germ Cells |
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