Subcellular identification of angiotensin I/II- and angiotensin II (AT 1)-receptor-immunoreactivity in the central nervous system of rats

To gain insight into generating and transport mechanisms of angiotensin (ANG) in the brain the study was focused on the subcellular localization of ANG II and its AT 1-receptors in the hypothalamus of rats. The present paper demonstrates ANG II- and AT 1-receptor-immunolabelling at brain parenchyma...

Full description

Saved in:
Bibliographic Details
Published in:Brain research 2003-02, Vol.962 (1), p.92-104
Main Authors: Thomas, Martin Alexander, Fleissner, Gerta, Hauptfleisch, Stefan, Lemmer, Björn
Format: Article
Language:English
Subjects:
Anatomy
Angiotensin I - analysis
Angiotensin II
Angiotensin II (AT 1)-receptor
Angiotensin II - analysis
Animals
Axons - ultrastructure
Biological and medical sciences
Brain - cytology
Brain - ultrastructure
Brain parenchyma vessel
Central nervous system
Electron microscopy
Endoplasmic Reticulum - ultrastructure
Endothelium - cytology
Endothelium - ultrastructure
Fundamental and applied biological sciences. Psychology
Hypothalamus
Immunocytochemistry
Immunohistochemistry
In Vitro Techniques
Nerve Endings - ultrastructure
Neurons - cytology
Neurons - ultrastructure
Rats
Receptor, Angiotensin, Type 1
Receptors, Angiotensin - analysis
Receptors, Angiotensin - physiology
Vertebrates: nervous system and sense organs
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To gain insight into generating and transport mechanisms of angiotensin (ANG) in the brain the study was focused on the subcellular localization of ANG II and its AT 1-receptors in the hypothalamus of rats. The present paper demonstrates ANG II- and AT 1-receptor-immunolabelling at brain parenchyma vessels and at glial and neuronal structures in the perivascular region. Further, ANG II- and AT 1-receptor-immunoreactivity is shown at plasma membranes and intracellular structures in the ependyma of the third ventricle. Based upon a conventional horseradish peroxidase technique, combined with the classical substrate 3,3′-diaminobenzidine, a procedure is introduced that will be useful with a variety of antibodies used on glutar- and paraformaldehyde-fixed brain tissue. This technique enables a fast correlation between light and electron microscopical results and might also provide an attractive alternative to colloidal gold-labelling and silver-intensification techniques.
ISSN:0006-8993
1872-6240
DOI:10.1016/S0006-8993(02)03971-9