Acetylation and level of mitochondrial transcription factor A in several organs of young and old rats

To gain further information on the role of mitochondrial transcription factor A (TFAM) in mitochondrial biogenesis, we studied the post-translational modifications of the protein in 6- and 28-month-old rat liver. Mass spectrometry and immunoblot analysis revealed that TFAM was acetylated at a single...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2003-01, Vol.301 (1), p.187-191
Main Authors: Dinardo, Maria M, Musicco, Clara, Fracasso, Flavio, Milella, Francesco, Gadaleta, Maria N, Gadaleta, Gemma, Cantatore, Palmiro
Format: Article
Language:English
Subjects:
Acetylation
Aging
Aging - physiology
Animals
Cerebellum - physiology
Copy number
DNA, Mitochondrial - metabolism
DNA-Binding Proteins
Heart - physiology
Kidney - physiology
Liver - physiology
Male
Mitochondria - metabolism
Mitochondrial Proteins
mtDNA
Nuclear Proteins - metabolism
Protein Processing, Post-Translational
Rats
Rats, Wistar
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
TFAM
Tissue Distribution
Transcription Factors - metabolism
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Summary:To gain further information on the role of mitochondrial transcription factor A (TFAM) in mitochondrial biogenesis, we studied the post-translational modifications of the protein in 6- and 28-month-old rat liver. Mass spectrometry and immunoblot analysis revealed that TFAM was acetylated at a single lysine residue and that the level of acetylation did not change with age. The measurement of the content of TFAM and of mitochondrial DNA (mtDNA) in several organs (cerebellum, heart, kidney, and liver) of young and old rats showed an age-related increase of mtDNA and TFAM in all the organs analyzed, except in heart. These data are discussed in the light of the multiple roles of TFAM in mitochondrial biogenesis and of the age-related change of the mitochondrial transcription.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(02)03008-5