Vascular networks and endothelial cells in the rat experimental pituitary glands and in the human pituitary adenomas

There has been considerable interest in the relationship between hormone‐secreting endocrine cells (HSEC) and their microvessels (MVN) in human pituitary gland. However, microcirculatory networks have rarely been studied in three dimensions (3D). Therefore, this study was designed to visualize and t...

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Bibliographic Details
Published in:Microscopy research and technique 2003-02, Vol.60 (2), p.231-235
Main Authors: Itoh, Johbu, Serizawa, Akihiko, Kawai, Kenji, Ishii, Yudo, Teramoto, Akira, Osamura, Robert Yoshiyuki
Format: Article
Language:English
Subjects:
3D-analysis CLSM
Adenoma - blood supply
Adenoma - metabolism
Adrenocorticotropic Hormone - metabolism
Animals
Antigens, CD34 - metabolism
Endothelium, Vascular - cytology
Female
Growth Hormone - metabolism
hormone-secreting endocrine cells
Hormones - metabolism
Hormones - secretion
human pituitary adenomas
Humans
Immunohistochemistry
Microcirculation - cytology
Microcirculation - drug effects
Microscopy, Confocal
microvessel network
pituitary gland
Pituitary Gland - blood supply
Pituitary Gland - cytology
Pituitary Gland - metabolism
Pituitary Neoplasms - blood supply
Pituitary Neoplasms - metabolism
Prolactin - metabolism
Rats
Rats, Wistar
vascular endothelial cells
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Summary:There has been considerable interest in the relationship between hormone‐secreting endocrine cells (HSEC) and their microvessels (MVN) in human pituitary gland. However, microcirculatory networks have rarely been studied in three dimensions (3D). Therefore, this study was designed to visualize and to reveal the relationship between hormone secreting endocrine cells and their microvessel environment including vascular endothelial cells in 3D using rat pituitary glands under various experimental conditions by confocal laser scanning microscopy (CLSM). By CLSM, the 3D distributions of MVN were visualized and revealed a relationship between HSEC and MVN in experimental pituitary glands and human pituitary adenomas. Therefore, 3D reconstructed imaging by CLSM is a useful technique with which to investigate the microvessel environment of hormone‐secreting cells and has the potential to reveal dynamic hormone‐secreting pathways. Microsc. Res. Tech. 60:231–235, 2003. © 2003 Wiley‐Liss, Inc.
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.10261