Proteoglycans in human burn hypertrophic scar from a patient with Ehlers-Danlos syndrome

• Published in: Carbohydrate research 1992, Vol.223, p.209-220
• Main Authors: Garg, Hari G., Lippay, Eric W., Neame, Peter J.
• Format: Article
• Language: English
• Subjects: Amino Acids - analysis; Biological and medical sciences; Burns - complications; Burns - metabolism; Carbohydrate Sequence; Child; Chromatography, DEAE-Cellulose; Chromatography, Ion Exchange; Cicatrix - metabolism; Cicatrix - pathology; Ehlers-Danlos Syndrome - complications; Ehlers-Danlos Syndrome - metabolism; Electrophoresis, Cellulose Acetate; Glycosaminoglycans - isolation & purification; Humans; Hypertrophy; Male; Medical sciences; Molecular Sequence Data; Molecular Weight; Proteoglycans - chemistry; Proteoglycans - isolation & purification; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/0008-6215(92)80017-U

Proteoglycans(PGs) from human burn hypertrophic scar of a patient with Ehlers-Danlos syndrome were extracted with 4 m guanidinium chloride and purified by DEAE-cellulose chromatography. Differential ethanol precipitation of the PG fraction obtained after ion-exchange chromatography yielded two low mol.-wt. PGs, one rich in glucuronic acid (PG GLCA; M r 66 kDa) and the other rich in iduronic acid (PG IDOA; M r 48 kDa). In PG GLCA, 84% of the glycosaminoglycan chains are composed of GlcA→GalNAc(SO 4) units, whereas in PG IDOA, the chains contain 95% IdoA→GalNAc(SO 4) disaccharide units. Upon treatment with testicular hyaluronidase, the PGs gave different-sized oligosaccharides. Chondroitinase ABC digestion of PG GLCA or PG IDOA gave a single protein core M r ∼20 kDa). The presence of glucosamine and sialic acid in PG GLCA and PG IDOA suggests that both contain N-linked oligosaccharides.