Bre1, an E3 Ubiquitin Ligase Required for Recruitment and Substrate Selection of Rad6 at a Promoter

Ubiquitination of histone H2B catalyzed by Rad6 is required for methylation of histone H3 by COMPASS. We identified Bre1 as the probable E3 for Rad6's role in transcription. Bre1 contains a C3HC4 (RING) finger and is present with Rad6 in a complex. The RING finger of Bre1 is required for ubiqui...

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Bibliographic Details
Published in:Molecular cell 2003, Vol.11 (1), p.267-274
Main Authors: Wood, Adam, Krogan, Nevan J, Dover, Jim, Schneider, Jessica, Heidt, Jonathan, Boateng, Marry Ann, Dean, Kimberly, Golshani, Ashkan, Zhang, Yi, Greenblatt, Jack F, Johnston, Mark, Shilatifard, Ali
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Gene Silencing
Histones - metabolism
Ligases - genetics
Ligases - metabolism
Lysine - metabolism
Methylation
Molecular Sequence Data
Open Reading Frames
Promoter Regions, Genetic
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Sequence Alignment
Telomere - metabolism
Ubiquitin - metabolism
Ubiquitin-Conjugating Enzymes
Ubiquitin-Protein Ligases
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Summary:Ubiquitination of histone H2B catalyzed by Rad6 is required for methylation of histone H3 by COMPASS. We identified Bre1 as the probable E3 for Rad6's role in transcription. Bre1 contains a C3HC4 (RING) finger and is present with Rad6 in a complex. The RING finger of Bre1 is required for ubiquitination of histone H2B, methylation of lysine 4 and 79 of H3 and for telomeric silencing. Chromatin immunoprecipitation experiments indicated that both Rad6 and Bre1 are recruited to a promoter. Bre1 is essential for this recruitment of Rad6 and is dedicated to the transcriptional pathway of Rad6. These results suggest that Bre1 is the likely E3 enzyme that directs Rad6 to modify chromatin and ultimately to affect gene expression.
ISSN:1097-2765
1097-4164
DOI:10.1016/S1097-2765(02)00802-X