The adjuvant OM-174 induces both the migration and maturation of murine dendritic cells in vivo

• Published in: Vaccine 2003-02, Vol.21 (9), p.836-842
• Main Authors: Pajak, Bernard, Garze, Virginie, Davies, Gwyn, Bauer, Jacques, Moser, Muriel, Chiavaroli, Carlo
• Format: Article
• Language: English
• Subjects: Adjuvant; Adjuvants, Immunologic - administration & dosage; Adjuvants, Immunologic - pharmacology; Animal vaccines; Animals; Antigen-Presenting Cells - classification; Antigen-Presenting Cells - cytology; Antigen-Presenting Cells - drug effects; Antigen-Presenting Cells - immunology; Biological and medical sciences; Cell Differentiation - drug effects; Cell Movement - drug effects; Dendritic cells; Dendritic Cells - classification; Dendritic Cells - cytology; Dendritic Cells - drug effects; Dendritic Cells - immunology; E coli; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Immune response; Immune system; Immunobiology; Injections, Intravenous; Injections, Subcutaneous; Laboratories; Langerhans Cells - classification; Langerhans Cells - cytology; Langerhans Cells - drug effects; Langerhans Cells - immunology; Lipids; Lipopolysaccharides - administration & dosage; Lipopolysaccharides - pharmacology; Lymph nodes; Lymph Nodes - cytology; Lymph Nodes - drug effects; Lymph Nodes - immunology; Lymphocytes; Maturation and migration; Mice; Mice, Inbred BALB C; Migration; Modulation of the immune response (stimulation, suppression); Rodents; Skin; Spleen; Spleen - cytology; Spleen - drug effects; Spleen - immunology
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(02)00529-7

The aim of this study was to test the capacity of the novel adjuvant OM-174, a lipid A analog, to induce the migration and the maturation of murine dendritic cells (DC) in vivo, a step which is considered as the initiation of the adaptive immune response. BALB/c mice were injected intravenously or subcutaneously with OM-174. The spleen and popliteal lymph nodes were harvested, and analyzed for DC localization and phenotype. The data presented here clearly show that, OM-174 induces the migration of DC from the periphery to the T cell areas of lymphoid organs, and their maturation into cells expressing high levels of MHC class II and co-stimulatory molecules, with a potency close to that of Escherichia coli lipopolysaccharide (LPS).