Benign Prostate-specific Antigen (BPSA) in Serum Is Increased in Benign Prostate Disease

BPSA is a "benign" form of free prostate-specific antigen (PSA) that is increased in prostate transition zone tissues of men with pathologic benign prostatic hyperplasia (BPH). We developed an immunoassay to determine the concentration of BPSA in the serum of men with BPH. The BPSA antigen...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 2003-02, Vol.49 (2), p.253-259
Main Authors: Linton, Harry J, Marks, Leonard S, Millar, Lisa S, Knott, Christine L, Rittenhouse, Harry G, Mikolajczyk, Stephen D
Format: Article
Language:English
Subjects:
Adult
Animals
Antigens
Biological and medical sciences
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Female
Genital system. Mammary gland
Humans
Hyperplasia
Immunoassay
Investigative techniques, diagnostic techniques (general aspects)
Liquid chromatography
Male
Medical sciences
Mice
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Plasma
Prostate cancer
Prostate-Specific Antigen - blood
Prostatic Hyperplasia - diagnosis
Prostatic Neoplasms - diagnosis
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Summary:BPSA is a "benign" form of free prostate-specific antigen (PSA) that is increased in prostate transition zone tissues of men with pathologic benign prostatic hyperplasia (BPH). We developed an immunoassay to determine the concentration of BPSA in the serum of men with BPH. The BPSA antigen was purified by HPLC, and murine monoclonal antibodies were prepared by standard methods. A fluorogenic ELISA was developed with high specificity for BPSA and no cross-reactivity with other forms of PSA. The BPSA immunoassay had a lower limit of detection of 6 ng/L and a cross-reactivity of
ISSN:0009-9147
1530-8561
DOI:10.1373/49.2.253