Induction of Chromatid Breaks by Carbon K-Shell Ultrasoft X Rays

• Published in: Radiation research 2003-02, Vol.159 (2), p.247-250
• Main Authors: Bryant, Peter E., Jones, Claire, Armstrong, Graham, Frankenberg-Schwager, Marlis, Frankenberg, Dieter
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Carbon; Carbon - chemistry; Cell lines; CHO Cells; Chromatids; Chromatids - drug effects; Chromatids - genetics; Chromatids - metabolism; Chromatids - radiation effects; Cobalt Radioisotopes - chemistry; Cricetinae; Cylinders; DNA Damage - drug effects; DNA Damage - radiation effects; Electrons; Gamma Rays; Medical sciences; Metal foils; Metaphase - radiation effects; Photons; Radiation dosage; Radiometry; REGULAR ARTICLES; Solar X rays; Space life sciences; X-Rays
• ISSN: 0033-7587; 1938-5404
• DOI: 10.1667/0033-7587(2003)159[0247:IOCBBC]2.0.CO;2

Bryant, P. E., Jones, C., Armstrong, G., Frankenberg-Schwager, M. and Frankenberg, D. Induction of Chromatid Breaks by Carbon K-Shell Ultrasoft X Rays. Radiat. Res. 159, 247–250 (2003). Chromatid breaks have previously been shown to be induced in G2-phase cells after exposure to ionizing radiation (X and γ rays) as a linear function of dose, consistent with a single-event mechanism. DNA double-strand breaks (DSBs) are thought to be the initiating lesion, and experiments with a genetically engineered cell line containing a single DSB site also indicate that a single DSB is sufficient to induce a chromatid break. Although the precise mechanism of conversion of an isolated DSB into a chromatid break is not yet understood, it is known that a proportion of chromatid breaks result from rearrangements between sister chromatids. Here we report further evidence for the single-event hypothesis for the formation of chromatid breaks. The evidence derives from experiments in which chromatid breaks have been induced by exposure of Chinese hamster cells to ultrasoft carbon K-shell X rays. Since the energy of carbon K-shell X rays is not sufficient for the secondary electrons to span more than one DNA double helix, we conclude that single traversals, and hence single (complex) DSBs, are responsible for the formation of chromatid breaks. We find that, as for 60Co γ rays, around 10% of the carbon K-shell X-ray-induced chromatid breaks have associated color switches at breakpoints, indicating that they arise through sister chromatid rearrangements.