Specific stimulation of MHC-transgenic mouse T-cell hybridomas with xenogeneic APC

From the recombinant human leukocyte antigen (HLA)-DR1/H2-E k major histocompatibility complex (MHC) class II–transgenic mice, we have generated two CD4 + T-cell hybridomas specific for peptides which were derived from human prostatic acid phosphatase (PAP) complexed to the human class II molecule H...

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Bibliographic Details
Published in:Human immunology 2003-02, Vol.64 (2), p.238-244
Main Authors: Vidovic, Damir, Graddis, Thomas J, Stepan, Lara P, Zaller, Dennis M, Laus, Reiner
Format: Article
Language:English
Subjects:
accessory molecules
Acid Phosphatase
Animals
Antigen Presentation
antigen presenting cells
Antigen-Presenting Cells - immunology
Antigens, Heterophile - immunology
CD4-Positive T-Lymphocytes - immunology
CD4-Positive T-Lymphocytes - metabolism
Cell Line - immunology
coreceptors
costimulation
Epitopes, T-Lymphocyte - immunology
Granulocyte-Macrophage Colony-Stimulating Factor - genetics
Granulocyte-Macrophage Colony-Stimulating Factor - immunology
H-2 Antigens - genetics
H-2 Antigens - immunology
HLA-DR1 Antigen - genetics
HLA-DR1 Antigen - immunology
Humans
Hybridomas - immunology
Interleukin-2 - metabolism
Mice
Mice, Transgenic
Protein Tyrosine Phosphatases - immunology
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - immunology
Recombinant Proteins - immunology
Species Specificity
T cell hybridomas
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Summary:From the recombinant human leukocyte antigen (HLA)-DR1/H2-E k major histocompatibility complex (MHC) class II–transgenic mice, we have generated two CD4 + T-cell hybridomas specific for peptides which were derived from human prostatic acid phosphatase (PAP) complexed to the human class II molecule HLA-DR1. Both hybridomas strongly react to PAP-pulsed antigen-presenting cells (APC) from transgenic mice. Interestingly, these hybridomas also responded to PAP antigen presented by HLA-DR1–positive human APC. The species-mismatched T-cell stimulation occurs despite the biologic discordance in participating accessory molecules, which are required for the optimal T-cell–APC interaction. Our results demonstrate various degrees of functional interaction between coreceptors, costimulatory molecules, and integrins, which are expressed on the surface of T-cell hybridomas and heterologous APC.
ISSN:0198-8859
1879-1166
DOI:10.1016/S0198-8859(02)00780-2