Secreted Antigens of Mycobacterium tuberculosis: Characterization with T Lymphocytes from Patients and Contacts after Two-Dimensional Separation

Little is known about T cell antigens involved in immunity against Mycobacterium tuberculosis. Most model systems use in vitro culture of human T lymphocytes with bacterial Iysates or secreted proteins as antigens. In this study, proteins from 3-week-old M. tuberculosis culture filtrates were separa...

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Bibliographic Details
Published in:The Journal of infectious diseases 1992-07, Vol.166 (1), p.186-190
Main Authors: Daugelat, Sabine, Guile, Heinz, Schoel, Bernd, Kaufmann, Stefan H.E.
Format: Article
Language:English
Subjects:
Antigens
Antigens, Bacterial - immunology
Bacillus calmette guerin vaccine
Biological and medical sciences
Cells, Cultured
Concise Comunications
Electrophoresis, Gel, Two-Dimensional
Gels
Humans
Immunity
Immunoblotting
Infections
Infectious diseases
Interleukin-2 - immunology
Isoelectric Focusing
Medical sciences
Mycobacterium tuberculosis
Mycobacterium tuberculosis - immunology
Recombinant Proteins - immunology
T lymphocytes
T-Lymphocytes - immunology
Tuberculin
Tuberculin Test
Tuberculosis
Tuberculosis vaccine
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Summary:Little is known about T cell antigens involved in immunity against Mycobacterium tuberculosis. Most model systems use in vitro culture of human T lymphocytes with bacterial Iysates or secreted proteins as antigens. In this study, proteins from 3-week-old M. tuberculosis culture filtrates were separated by two-dimensional PAGE and subsequently transferred into soluble phase. The resulting 480 fractions were screened with T lymphocytes from tuberculosis patients and healthy contacts. T cells from all 9 patients and from 8 of 10 tuberculin-positive contacts preferentially responded to a cluster of acidic proteins (pI 4–5) with molecular masses of 30–100 kDa, although they also recognized a number of other fractions. In contrast, of 7 tuberculin-negative contacts, 4 were not and 3 were only weakly stimulated by this cluster region. Therefore, this distinct cluster of secreted proteins seems to comprise dominant T cell antigens of M. tuberculosis.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/166.1.186