Enhanced expression of Runx2/PEBP2alphaA/CBFA1/AML3 during fracture healing

The cause of the dramatic increase in expression of the osteopontin gene during fracture healing was studied in a mouse experimental model. Semiquantitative reverse transcription-polymerase chain reaction, Northern blotting, and in situ hybridization analysis showed that the enhanced expression took...

Full description

Saved in:
Bibliographic Details
Published in:Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association 2003, Vol.8 (1), p.102-108
Main Authors: Kawahata, Hirohisa, Kikkawa, Toru, Higashibata, Yuji, Sakuma, Takahiko, Huening, Martin, Sato, Motohiko, Sugimoto, Mizuo, Kuriyama, Kohji, Terai, Kunihiro, Kitamura, Yukihiko, Nomura, Shintaro
Format: Article
Language:English
Subjects:
Animals
Blotting, Northern
Bone Morphogenetic Protein 2
Bone Morphogenetic Protein 4
Bone Morphogenetic Proteins - metabolism
Collagen Type I - metabolism
Core Binding Factor Alpha 1 Subunit
Fracture Healing - physiology
In Situ Hybridization
Male
Mice
Mice, Inbred ICR
Neoplasm Proteins
Osteopontin
Proto-Oncogene Protein c-ets-1
Proto-Oncogene Proteins - metabolism
Proto-Oncogene Proteins c-ets
Reverse Transcriptase Polymerase Chain Reaction
Sialoglycoproteins - genetics
Transcription Factors - metabolism
Transforming Growth Factor beta
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The cause of the dramatic increase in expression of the osteopontin gene during fracture healing was studied in a mouse experimental model. Semiquantitative reverse transcription-polymerase chain reaction, Northern blotting, and in situ hybridization analysis showed that the enhanced expression took place prior to callus formation. The change in the expression pattern of collagenous and noncollagenous bone matrix proteins in addition to Ets-1 and Runx2, major transcription factors of osteopontin, were examined and compared to that of osteopontin. Although Ets-1 expression showed no significant change during fracture healing, enhanced expression of Runx2 corresponding to that of osteopontin was observed. Furthermore, in situ hybridization demonstrated that osteopontin-expressing cells also express the Runx2 gene. The results indicated the possibility that Runx2 is a major regulator of osteopontin during fracture healing.
ISSN:0949-2658