Membrane chromatographic method for the rapid purification of vitellogenin from fish plasma

A membrane chromatographic method was developed for the rapid purification of vitellogenin (Vtg) from the plasma of 17β-estradiol induced loach ( Misgurnus angaillicaud atus) and carp ( Cyprinus carpio). The time required for the proposed procedure is less then 10 min at a flow-rate of 5 ml/min of t...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2003-03, Vol.785 (2), p.361-368
Main Authors: Shi, Guoqing, Shao, Jing, Jiang, Guibin, Wang, Qianxing, Lu, Yiqiang, Liu, Jingfu, Liu, Jiemin
Format: Article
Language:English
Subjects:
Animals
Carps - metabolism
Chromatography
Chromatography, DEAE-Cellulose
Chromatography, Ion Exchange
Cypriniformes - metabolism
Electrophoresis, Polyacrylamide Gel
Estradiol - pharmacology
Female
Indicators and Reagents
Male
Membranes, Artificial
Proteins - chemistry
Vitellogenin
Vitellogenins - blood
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Summary:A membrane chromatographic method was developed for the rapid purification of vitellogenin (Vtg) from the plasma of 17β-estradiol induced loach ( Misgurnus angaillicaud atus) and carp ( Cyprinus carpio). The time required for the proposed procedure is less then 10 min at a flow-rate of 5 ml/min of the mobile phase, and 0.5 ml of fish plasma could be separated in one cycle. Multistep gradient elution was more suitable for the separation than linear gradient elution. Under optimized conditions, a single Vtg peak can be obtained and its identity was confirmed by SDS–PAGE and gel-permeation chromatography assessment. This method is rapid and easy to operate compared to conventional HPLC and FPLC columns for Vtg separation.
ISSN:1570-0232
1873-376X
DOI:10.1016/S1570-0232(02)00958-3