Pax6 in the sepiolid squid Euprymna scolopes: evidence for a role in eye, sensory organ and brain development

• Published in: Mechanisms of development 2003-02, Vol.120 (2), p.177-183
• Main Authors: Hartmann, B, Lee, P.N, Kang, Y.Y, Tomarev, S, de Couet, H.G, Callaerts, P
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Brain; Brain - embryology; Brain - metabolism; Cell Nucleus - metabolism; Cloning, Molecular; Decapodiformes - embryology; Decapodiformes - genetics; Development; Embryo, Nonmammalian; Euprymna scolopes; Eye; Eye - embryology; Eye - metabolism; Eye Proteins - genetics; Eye Proteins - metabolism; Gene Expression Regulation, Developmental; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; Molecular Sequence Data; Paired Box Transcription Factors; Pax6; PAX6 Transcription Factor; Repressor Proteins; Sense Organs - embryology; Sense Organs - metabolism; Sensory organ; Sequence Homology, Amino Acid; Squid
• ISSN: 0925-4773; 1872-6356
• DOI: 10.1016/S0925-4773(02)00456-2

The cloning of a Pax6 orthologue from the sepiolid squid Euprymna scolopes and its developmental expression pattern are described. The data are consistent with the presence of a single gene encoding a protein with highly conserved DNA-binding paired and homeodomains. A detailed expression analysis by in situ hybridization and immunodetection revealed Pax6 mRNA and protein with predominantly nuclear localization in the developing eye, olfactory organ, brain lobes (optic lobe, olfactory lobe, peduncle lobe, superior frontal lobe and dorsal basal lobe), arms and mantle, suggestive of a role in eye, brain, and sensory organ development.