Cell growth effects of Epstein--Barr virus leader protein

• Published in: Journal of general virology 1992-06, Vol.73 (6), p.1547-1551
• Main Authors: Allan, Gordon J, Inman, Gareth J, Parker, Bruce D, Rowe, David T, Farrell, Paul J
• Format: Article
• Language: English
• Subjects: Antigens, Viral - genetics; B-Lymphocytes - cytology; B-Lymphocytes - microbiology; Biological and medical sciences; Blotting, Western; Cell Division; Cell Line, Transformed; Cell Transformation, Viral; Epstein-Barr virus; Epstein-Barr Virus Nuclear Antigens; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Herpesvirus 4, Human - genetics; Herpesvirus 4, Human - immunology; Herpesvirus 4, Human - physiology; Humans; Microbiology; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; Transfection; Virology
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-73-6-1547

Ludwig Institute for Cancer Research, St Mary's Hospital Medical School, Norfolk Place, London W2 1PG, U.K. B lymphoblastoid cell lines immortalized with P3HR1/633 Epstein—Barr virus (EBV), which has a deletion in the EBV nuclear antigen leader protein (EBNA-LP) gene, were transfected with a vector expressing wild-type EBNA-LP. The EBNA-LP trans-fectants grew out faster under G418 selection than control cells but expression of EBNA-LP made no significant difference to growth rate or saturation density of the resulting established cell lines. When the cells expressing EBNA-LP were allowed to grow to saturation and then diluted in fresh medium they underwent DNA synthesis more rapidly than control cultures. St Judes Children's Research Hospital, 332 North Lauderdale, Memphis, Tennessee 38101-0318, U.S.A. > Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, U.S.A. Received 4 December 1991; accepted 10 February 1992.