Candidate markers for stem and early progenitor cells, Musashi-1 and Hes1, are expressed in crypt base columnar cells of mouse small intestine

Musashi-1, a neural RNA-binding protein, is important for maintaining neural stem cells. Both Musashi-1 and Hes1, a transcriptional factor regulated by Musashi-1, are expressed in the small intestine. Here we show that Musashi-1 is present in a few epithelial cells just above the Paneth cells in the...

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Bibliographic Details
Published in:FEBS letters 2003-01, Vol.535 (1-3), p.131-135
Main Authors: Kayahara, Takahisa, Sawada, Mitsutaka, Takaishi, Shigeo, Fukui, Hirokazu, Seno, Hiroshi, Fukuzawa, Hiroaki, Suzuki, Katsumasa, Hiai, Hiroshi, Kageyama, Ryoichiro, Okano, Hideyuki, Chiba, Tsutomu
Format: Article
Language:English
Subjects:
(RT-)PCR, (reverse transcription-)polymerase chain reaction
Animals
Basic Helix-Loop-Helix Transcription Factors
Biomarkers - analysis
Cell Line
Crypt base columnar cell
GAPDH, glyceraldehyde-3-phosphate dehydrogenase
Hes1
Homeodomain Proteins - biosynthesis
Homeodomain Proteins - genetics
Humans
Intestine, Small - cytology
Intestine, Small - growth & development
Intestine, Small - metabolism
Mice
Mice, Inbred ICR
Msi-1, Musashi-1
Musashi-1
Nerve Tissue Proteins - biosynthesis
Nerve Tissue Proteins - genetics
Organ Specificity
Paneth Cells - cytology
Rats
RNA, Messenger - biosynthesis
RNA-Binding Proteins - biosynthesis
RNA-Binding Proteins - genetics
Small intestine
Stem cell
Stem Cells - cytology
Stem Cells - metabolism
Transcription Factor HES-1
Transcription Factors - biosynthesis
Transcription Factors - genetics
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Summary:Musashi-1, a neural RNA-binding protein, is important for maintaining neural stem cells. Both Musashi-1 and Hes1, a transcriptional factor regulated by Musashi-1, are expressed in the small intestine. Here we show that Musashi-1 is present in a few epithelial cells just above the Paneth cells in the small intestinal crypt, the putative position of stem cells, whereas Hes1 is expressed in lower crypt cells just above the Paneth cells, including Musashi-1-positive cells. Musashi-1 and Hes1 were not expressed in Paneth cells. Notably, Musashi-1 and Hes1 were coexpressed in the crypt base columnar cells located between the Paneth cells. These findings suggest that not only the cells just above Paneth cells but also the crypt base columnar cells between the Paneth cells have stem cell characteristics.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(02)03896-6