DNA immunization with pgp3 gene of Chlamydia trachomatis inhibits the spread of chlamydial infection from the lower to the upper genital tract in C3H/HeN mice

• Published in: Vaccine 2003-03, Vol.21 (11), p.1089-1093
• Main Authors: Donati, Manuela, Sambri, Vittorio, Comanducci, Maurizio, Di Leo, Korinne, Storni, Elisa, Giacani, Lorenzo, Ratti, Giulio, Cevenini, Roberto
• Format: Article
• Language: English
• Subjects: Animals; Antigens, Bacterial - genetics; Antigens, Bacterial - immunology; Bacterial Proteins - genetics; Bacterial Proteins - immunology; Bacteriology; Biological and medical sciences; Chlamydia Infections - drug therapy; Chlamydia Infections - immunology; Chlamydia trachomatis; Chlamydia trachomatis - genetics; Chlamydia trachomatis - immunology; Disease Progression; Female; Fundamental and applied biological sciences. Psychology; Genetic Vectors - genetics; Immunization; Immunotherapy, Active; Mice; Mice, Inbred C3H; Microbiology; Mouse genital infection; Pelvic Inflammatory Disease - microbiology; Pelvic Inflammatory Disease - prevention & control; pgp3 DNA immunization; Salpingitis - immunology; Salpingitis - microbiology; Salpingitis - prevention & control; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, DNA - immunology; Vaccines, DNA - therapeutic use; Vaginosis, Bacterial - immunology; Vaginosis, Bacterial - therapy
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(02)00631-X

Chlamydia trachomatis pgp3 DNA immunized (no. 300) and non-immunized (no. 300) C3H/HeN mice were infected by vaginal inoculation with infectious C. trachomatis serotype D elementary bodies (EBs) and the spread of infection to the salpinges was assessed by cell culture isolation from tissue homogenates 7, 14, 21, 28, 35 and 42 days post-infection (p.i.). Overall, the pgp3-DNA immunization prevented salpinx infection in 94 (56%) mice, if compared with the 168 positive animals found among the non-immunized animals ( P