Use of DNA Probes to Detect Mycobacterium intracellulare and “X” Mycobacteria among Clinical Isolates of Mycobacterium avium Complex

• Published in: The Journal of infectious diseases 1992-07, Vol.166 (1), p.191-194
• Main Authors: Cregan, Patricia, Yajko, David M., Ng, Valerie L., Gonzalez, Patricia C., Nassos, Patricia S., Sanders, Cynthia A., Keith Hadley, W.
• Format: Article
• Language: English
• Subjects: Acquired Immunodeficiency Syndrome - microbiology; Adult; Aged; AIDS/HIV; American culture; Blood; Bronchoalveolar Lavage Fluid - microbiology; Cerebrospinal fluid; Concise Comunications; DNA Probes; DNA, Bacterial; Epidemiology; Female; HIV 1; Humans; Infections; Male; Microbiology; Middle Aged; Mycobacterium; Mycobacterium avium; Mycobacterium avium complex; Mycobacterium avium Complex - genetics; Mycobacterium avium Complex - isolation & purification; Mycobacterium avium-intracellulare Infection - microbiology; Mycobacterium intracellulare; Nucleic Acid Hybridization; Sputum - microbiology
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1093/infdis/166.1.191

A mycobacterial DNA probe (designated X) wasrecently developed to help identify Mycobacterium avium complex (MAC) isolates that are nonreactive with probes specific for M. avium or Mycobacterium intracellulare. The prevalence of X probe-positive mycobacteria in clinical specimens and their role in causing disease is unknown. Using a DNA probe kit that includes the X probe, we characterized 100 consecutive clinical MAC isolates as M. avium, M. intracellulare, or X. Lysates from 81 of the isolates reacted with the M. avium probe, 13 with the M. intracellulare probe, 3 with the X probe, and 3 failed to hybridize with any of the probes. All three X-positive isolates were recovered from sputa of patients who were recent immigrants to the United States and who presented with hemoptysis. One isolate was from a Hispanic man infected with human immunodeficiency virus type 1 (HIV-1) and the other 2 were from Filipino patients with no HIV-1 risk factors. This study also showed a higher than expected number of M. intracellulare isolates from blood and cerebrospinal fluid of HIV-I-infected patients.