Molecular Analysis of Virus-Producing and Non-Producing Clones Derived from a Defective SSPE Virus Yamagata-1 Strain

Two virus clones were isolated from a defective SSPE virus, the Yamagata-1 strain, and designated as the YA and YF clones. The YA clone-infected cells produced neither cell-free virus nor cell-associated virus, whereas the YF cloneinfected cells produced both cell-associated and cell-free virus. No...

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Published in:MICROBIOLOGY and IMMUNOLOGY 1992, Vol.36(3), pp.257-267
Main Authors: Haga, Takeshi, Komase, Katsuhiro, Yoshikawa, Yasuhiro, Yamanouchi, Kazuya
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies, Monoclonal
Base Sequence
Biological and medical sciences
Blotting, Western
Clone Cells
DNA, Viral - genetics
Epitopes
Fundamental and applied biological sciences. Psychology
Hemadsorption Inhibition Tests
Microbiology
Molecular Sequence Data
Morphology, structure, chemical composition, physicochemical properties
RNA, Messenger - genetics
SSPE Virus - genetics
Vero Cells
Viral Structural Proteins - analysis
Virology
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Summary:Two virus clones were isolated from a defective SSPE virus, the Yamagata-1 strain, and designated as the YA and YF clones. The YA clone-infected cells produced neither cell-free virus nor cell-associated virus, whereas the YF cloneinfected cells produced both cell-associated and cell-free virus. No difference of epitopes on structural proteins was observed between these two clones. Both clones had hemadsorption activity. Quantitation of structural protein by Western dot blots showed relatively a lower amount of M protein in the YA-infected cells than that in the YF-infected cells. The ratio, P plus M dicistronic/M monocistronic mRNA, in the YA-infected cells was about twice that in the YF-infected cells. Sequence analysis of cDNA corresponding to P plus M dicistronic mRNA revealed that the deduced M protein of the YF virus was smaller than that of the YA virus by five amino acids from the carboxy terminal. These results suggest that abundant production of P plus M dicistronic mRNA is responsible for the reduced amount of M protein in the non-productive YA clone.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1992.tb01663.x