15N NMR Study on Site-Selective Binding of Metal Ions to Guanine Runs in DNA:  A Good Correlation with HOMO Distribution

15N NMR of DNA containing 15N-N7-enriched guanine (G) in the presence of paramagnetic ions (Mn(II) and Co(II)) was investigated. As the concentration of metal ion was increased, 15N NMR signals of the 5‘G of GG and the middle G of GGG broadened site-selectively, indicating that electron-donating sit...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2003-02, Vol.125 (5), p.1172-1173
Main Authors: Okamoto, Akimitsu, Kanatani, Keiichiro, Taiji, Toshiji, Saito, Isao
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Cations, Divalent
Cobalt - chemistry
DNA - chemistry
Dna, deoxyribonucleoproteins
Fundamental and applied biological sciences. Psychology
Guanine - chemistry
Manganese - chemistry
Nitrogen Isotopes
Nuclear Magnetic Resonance, Biomolecular - methods
Nucleic acids
Oligonucleotides - chemical synthesis
Oligonucleotides - chemistry
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Summary:15N NMR of DNA containing 15N-N7-enriched guanine (G) in the presence of paramagnetic ions (Mn(II) and Co(II)) was investigated. As the concentration of metal ion was increased, 15N NMR signals of the 5‘G of GG and the middle G of GGG broadened site-selectively, indicating that electron-donating sites in G runs preferentially localize on the 5‘G of GG and the middle G of GGG. The selectivity for G−metal ion interaction observed in this study was in good agreement with calculated HOMO distribution of G runs.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja0279388