Down-regulation of human protein kinase C α is associated with terminal neutrophil differentiation

We have established an RNase protection method to quantify the expression of mRNA for the human protein kinase C (PK-C) isoforms alpha, beta 1, beta 2, and gamma. This was used to investigate whether each isoform is differentially expressed during the differentiation of hematopoietic cells. Myeloid...

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Bibliographic Details
Published in:Blood 1992-07, Vol.80 (1), p.68-76
Main Authors: DEVALIA, V, THOMAS, S. B, ROBERTS, P. J, MARK JONES, H, LINCH, D. C
Format: Article
Language:English
Subjects:
Biological and medical sciences
Calcitriol - pharmacology
Cell Differentiation - drug effects
Cell differentiation, maturation, development, hematopoiesis
Cell physiology
Dimethyl Sulfoxide - pharmacology
Fundamental and applied biological sciences. Psychology
Gene Expression
Hematopoietic Stem Cells - physiology
Humans
In Vitro Techniques
Molecular and cellular biology
Monocytes - cytology
Monocytes - enzymology
Neutrophils - cytology
Neutrophils - enzymology
Protein Kinase C - genetics
Protein Kinase C - metabolism
RNA, Messenger - genetics
Tretinoin - pharmacology
Tumor Cells, Cultured
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Summary:We have established an RNase protection method to quantify the expression of mRNA for the human protein kinase C (PK-C) isoforms alpha, beta 1, beta 2, and gamma. This was used to investigate whether each isoform is differentially expressed during the differentiation of hematopoietic cells. Myeloid and lymphoid cells express PK-C alpha, beta 1, and beta 2 mRNAs in various proportions. PK-C gamma mRNA was detected in human brain, but not in hematopoietic cells. PK-C alpha mRNA decreases as HL-60 cells mature to a neutrophil phenotype in response to retinoic acid, but its abundance does not change during monocytic differentiation in response to vitamin D3. PK-C alpha mRNA and protein were undetectable in peripheral blood neutrophils, but are present in monocytes. The mRNAs for PK-C beta 1 and beta 2 isoforms increase during HL-60 differentiation and are expressed in both neutrophils and monocytes. Therefore, the PK-C alpha isoform is specifically down-regulated during human neutrophil terminal differentiation. These data suggest that mature neutrophil functions do not require the PK-C alpha isoform.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V80.1.68.68